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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Biodegradation of environmental pollutans - Crystallization haloalkan dehalogenase DpcA of Psychrobacter cryohalolentis K5
 JUNG, Jakub
Knowledge of protein structures is necessary to clarify their structure-function relationships. Haloalkane dehalogenases are microbial enzymes that catalyze the hydrolysis of carbon-halogen in the halogenated hydrocarbons. Based on their properties these enzymes are able to degrade halogenated compounds. As the environment is burdened by a lot of different pollutants, biodegradation is a necessary step to improve the quality of environment. Haloalkane dehalogenases can be used as biosensors since they can detect contamination by halogenated compounds. This thesis is focused on crystallization of the model protein lysozyme and haloalkane dehalogenase DpcA isolated from the organism Psychrobacter cryohalolentis K5. The aim of this work was to prepare crystals suitable for X-ray diffraction analysis. After verification of protein?s purity, both proteins were crystallized by the use of basic and advanced crystallization methods. Single crystals of suitable size and quality for X-ray diffraction analysis were obtained and diffraction data were used to solve the 3D atomic structure of the DpcA. Since haloalkane dehalogenases have a low activity for halogenated substrates, the main aim of protein engineering is to increase their activity so thus further research is focused on modification of substrate specificity.
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Dehalogenation of Polychlorinated Phenols
 Bureš, M. ; Pekárek, Vladimír
The aim of this study is thermodynamical study of dehalogenation of polychlorinated phenols in the gaseous state by means of steam in presence of solid copper. The purpose of this study is a thermodynamical interpretation of reaction mechanism of dehalogenation processes and comparison with the measured kinetics data.The work carried out with polychlorinated benzenes and is a part of more wide research program, dealing with dehalogenation of polychlorinated aromatic polutants.
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