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Conservation of Allium germplasm collection by cryopreservation
Zámečník, Jiří ; Grospietsch, Martin ; Kotková, Renata ; Faltus, Miloš
Allium is important crop in the Czech Republic. Allium sativum L. germplasm is maintained in the field collection and this fact increases risk of accidental lost of genotype. Conservation of Allium sativum L. germplasm by means cryopreservation decreases risks of genotype lost. Allium sativum L. samples are stored at ultralow temperature that stopped all biochemical processes and the samples are stored without any changes for many years. This methodology describes procedure of material preparation, explant preculture and dehydration by mixture of cryoprotectants, shoot tips cryopreservation and their recovery. Principles of safe genotype storage and recovery are defined. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize the Allium sativum L. cryopreservation method in the frame of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
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The Questions of the Beginning and the End of Human Life
ČADOVÁ, Marie
This work deals with the problems of conception, gravidity and abortion in the first part and in the second part than deals with problems of death and dying. It concerns two cut-off points, which are component parts of human life. The first part surveis the biginning of human life and tries to find answers for questions, which have a connection with the conception, artifical insemination, surrogative maternity and abortion. It describes paradoxes, which comes from such situations. It appreciates this paradoxes in the wiev of medicin, ethics, psychology and law. The second part describes the end of the human life in several forms. It broods killing, suicide and euthansia in aspects of the psychology, medicine, law and ethics. In the end it describes the palliative care as an alternativ to euthansia.
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