|
|
Study of plasma interaction with yeast Candida glabrata
Trebulová, Kristína ; Lehocký, Marián (referee) ; Krčma, František (advisor)
The main subject of this diploma thesis is the study of low temperature discharges´ effects on the yeast Candida glabrata (C. glabrata). Cold atmospheric-pressure plasma (CAPP) technology has achieved a great deal of attention in recent years, due to its positive attributes, including high efficiency against a wide range of microorganisms, ease of operation, economic simplicity and also environmental friendliness. The research of CAPP focuses mainly on the applications involving the treatment of cancer and superficial infections as well as sterilization technologies in medicine, food and agriculture. Various effects and also some mechanisms of inactivation of bacteria and tumor cells have been explained over the last decade but the effects and mechanisms of fungi inactivation have not yet been sufficiently investigated. The aim of this work is to verify the effects of chosen low-temperature discharges on the model yeast example C. glabrata, in order to establish basic plasma set-up for further studies and experiments in vivo and to expand a global knowledge about the antifungal effects of CAPP. In this study, 5 different discharges, namely: microwave (unipolar and surface wave) in the form of plasma torch, transient spark, corona discharge in the form of corona pen and dielectric barriere discharge (DBD) in the form of DBD pen were tested. All discharges were tested by direct application onto a 24-hour inoculum of C. glabrata inoculated on agar plates. The results were evaluated based on the size and stability of the inhibition zones formed in the post treatment cultivation. From the individual measurements optimal conditions for testing the inhibitory effects of low temperature discharges on the selected yeast were determined. The microwave discharges (unipolar and surface wave) were chosen to be the most effective in the inhibition of C. glabrata. The measurements were therefore pursued with these discharges under selected conditions corresponding to chosen application of sterilization of surface infections in living organisms. The unipolar microwave discharge was chosen to be the most effective discharge for the potential treatment of superficial candidiasis. The indirect effect of plasma was also investigated using plasma activated liquid (PAL) on inoculated yeast culture. PAL was prepared using 3 different discharges: Dielectric Barrier Discharge (DBD), Unipolar Microwave Discharge and Electric Discharge in Liquid. The results of this work prove significant inhibitory effects of CAPP on the yeast C. glabrata, within the limits of in vitro cultivation and the so-called flat biology. The overall inhibitory effects are directly proportional to the irradiation time, the magnitude of applied power and also the area covered by the discharge. In terms of the initial cell concentration an indirect dependency can be observed, but it can also be stated that this factor is less significant compared to the other examined factors. Observations of the effects of plasma activated liquid (PAL) indicate that a single application of PAL under selected conditions is not alone enough for successful inhibition of C. glabrata.
|
|
|
Study of plasma interaction with yeast Candida glabrata
Trebulová, Kristína ; Lehocký, Marián (referee) ; Krčma, František (advisor)
The main subject of this diploma thesis is the study of low temperature discharges´ effects on the yeast Candida glabrata (C. glabrata). Cold atmospheric-pressure plasma (CAPP) technology has achieved a great deal of attention in recent years, due to its positive attributes, including high efficiency against a wide range of microorganisms, ease of operation, economic simplicity and also environmental friendliness. The research of CAPP focuses mainly on the applications involving the treatment of cancer and superficial infections as well as sterilization technologies in medicine, food and agriculture. Various effects and also some mechanisms of inactivation of bacteria and tumor cells have been explained over the last decade but the effects and mechanisms of fungi inactivation have not yet been sufficiently investigated. The aim of this work is to verify the effects of chosen low-temperature discharges on the model yeast example C. glabrata, in order to establish basic plasma set-up for further studies and experiments in vivo and to expand a global knowledge about the antifungal effects of CAPP. In this study, 5 different discharges, namely: microwave (unipolar and surface wave) in the form of plasma torch, transient spark, corona discharge in the form of corona pen and dielectric barriere discharge (DBD) in the form of DBD pen were tested. All discharges were tested by direct application onto a 24-hour inoculum of C. glabrata inoculated on agar plates. The results were evaluated based on the size and stability of the inhibition zones formed in the post treatment cultivation. From the individual measurements optimal conditions for testing the inhibitory effects of low temperature discharges on the selected yeast were determined. The microwave discharges (unipolar and surface wave) were chosen to be the most effective in the inhibition of C. glabrata. The measurements were therefore pursued with these discharges under selected conditions corresponding to chosen application of sterilization of surface infections in living organisms. The unipolar microwave discharge was chosen to be the most effective discharge for the potential treatment of superficial candidiasis. The indirect effect of plasma was also investigated using plasma activated liquid (PAL) on inoculated yeast culture. PAL was prepared using 3 different discharges: Dielectric Barrier Discharge (DBD), Unipolar Microwave Discharge and Electric Discharge in Liquid. The results of this work prove significant inhibitory effects of CAPP on the yeast C. glabrata, within the limits of in vitro cultivation and the so-called flat biology. The overall inhibitory effects are directly proportional to the irradiation time, the magnitude of applied power and also the area covered by the discharge. In terms of the initial cell concentration an indirect dependency can be observed, but it can also be stated that this factor is less significant compared to the other examined factors. Observations of the effects of plasma activated liquid (PAL) indicate that a single application of PAL under selected conditions is not alone enough for successful inhibition of C. glabrata.
|
guest ::
