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Aspekty spermatologického vyšetření čerstvého ejakulátu beranů
Weberová, Gabriela
The diploma thesis deals with the ram genital organs, hormonal regulation, sperm production and ejaculate. Practically were made preparations of rams before collecting semen, semen collection and ways of obtain semen. Further were evaluated quality native ram semen by macroscopically and microscopically methods. Evaluated material was obtained from zwartbles rams breed owned by Ing. Martin Hošek, Ph.D. All the ejaculate was immediately after collection examined macroscopically and microscopically. The volume of ejaculate, wave movement and activity of sperm were evaluated. Subsequently ejaculate transported was to the laboratory department of livestock reproduction of the Department of Animal Breeding Faculty of Agronomy, Mendel University in Brno. There was also assessed the concentration of sperm was made chill survival test when ejaculate was stored at 3 °C and the results were evaluated after 24 and 48 hours. There were also evaluated morphological coatings, which monitored defects of the head, acrosome defects, defects in the flagellum, immature sperm cells, sperm cells degeneration and normal sperm. The results show that the volume of the obtained sperm has an effect on genetic line, season, frequency of colection and age groups. For semen quality were proven decisive sampling sequence which had the effect of sperm concentration and survivability.
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