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National Repository of Grey Literature

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Studying of contrast formation mechanisms in cryogenic scanning electron microscopy of life-science specimens Graubnerová, Eliška ; Vičar, Tomáš (referee) ; Nováček, Jiří (advisor) Tato bakalářská práce se zabývá fyzikálními jevy v elektronové mikroskopii, stavbou skenovacího elektronového mikroskopu a jeho fungováním. Je také popsána příprava vzorků a její specifika v kryogenní elektronové mikroskopii. Důležitou částí práce je popis vzniku obrazu a kontrastu, včetně nabíjecích a závěsových artefaktů. Experimentální část se věnuje přípravě lipidů a jejich zobrazování v kryogenním skenovacím elektronovém mikroskopu za různých zobrazovacích podmínek. Klade se důraz hlavně na studium kontrastu a nabíjecích artefaktů. Detailed record

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