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Změny hematologických parametrů při jarních úhynech kaprů
NÁDASKÝ, Alexandr
The aim of the bachelor thesis was to describe the effect of carp edema virus disease caused by carp edema virus on selected hematological factors of blood of infected common carp (Cyprinus carpio). Subsequently, the results of infected and uninfected fish were compared with each other. Twenty-eight carp were caught in the spring of 2019 from three different locations on suspicion of carp edema virus. During the autumn, another 30 carp were taken from identical localities. Fish from the autumn period were caught to test the persistence of viral DNA in gill tissue. Blood and gill tissue samples were taken from each tested fish. Total erythrocyte count (RBC), total leukocyte count (WBC), packed cell volume (PCV), amount of hemoglobin (Hb), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC) and differential white blood cell count were determined from blood samples. The results show that in CEV positive fish there was a statistically significant difference in total erythrocyte count (RBC), packed cell volume (PCV) and amount of hemoglobin (Hb). The values of these parameters increased compared to CEV negative fish. The only significant difference in the differential white blood cell count is an increase in the percentage of neutrophil bands in CEV-positive fish. However, it is important to note that a change in the value of haematological factors may occur not only due to CEVD infection. This is also evidenced by statistically significant difference between carp from the spring or autumn period and from individual localities. For these difference, it can be assumed that their difference was caused by influences such as different water quality, temperature, stocking density, reproductive cycle, toxic substances and pathogenic organisms.
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