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Utilization of vibrational spectroscopy in structural analysis of microbially produced alginates
 Kianička, Michal ; Enev, Vojtěch (referee) ; Sedláček, Petr (advisor)
This bachelor thesis focuses on the characterization of alginates using the main vibrational methods FTIR-ATR and Raman spectroscopy, supplemented by the carbazole method and thermogravimetry (TGA). The main objective was to utilize these analytical tools to study commercially available alginates and determine their monomeric composition. For this purpose, a calibration set was created using the ratios of poly(mannuronic acid) and poly(guluronic acid) standards, which was then used to analyze commercial alginates. The thesis focused on two forms of alginates - acidic and alkaline salts, aiming for a better understanding of their composition and subsequent application. The thesis revealed that the vibrational methods used were effective in analyzing the calibration sets, providing calibration curves for determining the composition of alginates. However, in the actual analysis of commercial alginates, these methods were found to be insufficiently accurate and reliable. Possible explanations include the presence of additional components in commercial samples or the influence of molecular weight. The results of the thesis indicate the need for further research tasks aimed at improving the accuracy and effectiveness of vibrational methods in the analysis of commercial alginates.
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