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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Modification of inner capillary surface for electrophoretic separations of liposomes
 Píplová, Renata ; Křížek, Tomáš (advisor) ; Čokrtová, Kateřina (referee)
Electroosmotic flow is one of the main factors that affect the resolution of separations in capillary electrophoresis and its modification is often necessary. One way of modification of the electroosmotic flow is the use of dynamic or permanent coating of the inner capillary surface. Such modifications during liposome separation can also reduce the tendency of liposomes to adsorb to the capillary surface. The aim of this work was to find a stable coating that will sufficiently eliminate the electroosmotic flow and to test its effect on the separation of liposomes. At first, the dynamic coatings of the capillary with Pluronic F-127, polyvinylpyrrolidone K30, polydiallyldimethylammonium chloride, polyethylene glycol and polyvinyl alcohol were tested using four different methods. Individual polymers and methods differed in the rate of elimination of the electroosmotic flow as well as in the stability of the capillary coatings. Dynamic coatings with Pluronic F-127, polyvinylpyrrolidone K30 and polydiallyldimethylammonium chloride were selected for further liposome separation testing. Fluorescently labeled liposomes were separated using capillary electrophoresis with laser-induced fluorescence detection and the mobilities of liposomes separated using capillaries with different coatings were determined....
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