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Calprotectin determination in patients with autoimmune disease.
ZÁZVORKOVÁ, Klára
The bachelor thesis deals with autoimmune diseases of GIT and laboratory determination of calprotectin by chemiluminescence method. In the theoretical part I focused on individual diseases, their description and treatment options. The main part of the thesis is devoted to the laboratory diagnosis of calprotectin from stool. I statistically evaluated 300 samples. And further I compared the method with other p-ANCA and ASCA methods.
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Biochemical predictors of the treatment failure in pediatric IBD patients
Zárubová, Kristýna ; Bronský, Jiří (advisor) ; Hrdý, Jiří (referee) ; Petrášová, Miroslava (referee)
Up to 20 % of patients with IBD (inflammatory bowel disease) is diagnosed in childhood, the course of the disease tends to often be more severe and the treatment more difficult than in case of adults. Therefore, it is necessary to commence or eventually change the treatment in order to prevent development of complications. Early detection of relapse plays significant role for further course of the disease and general prognosis of the patient. Changes to laboratory parameters (e.g. to the level of fecal calprotectin) can significantly prevent the development of clinical symptoms and may indicate the necessity for control endoscopy, further escalation or change in therapy. The primary focus of this thesis are patients who underwent surgical bowel resection, who constitute one of the risk groups. Studies show that as many as 70 % of patients suffering from Crohn's disease reach the point of bowel surgery within the first 10 years of the diagnosis. According to the results, a promising predictor of relapse of the disease seems to be serum albumin, which may relate to the overall nutritional status of the organism. We have also examined the effectivity of using fecal calprotectin and CRP as relapse predictors. Testing directly in the resected bowel did not prove usability of calprotectin as a predictor,...
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Ways to test for Helicobacter and calprotectin
LAVIČKOVÁ, Lucie
H. pylori is a gram-negative rod bacteria of a spiral shape. It is obligatory microaerofile. It has strong urease activity. It colonize the stomach mucosa and it is proved, that almost the half of human population is colonized. H. pylori concluding other factors can cause gastritis which can become chronic. Other diseases can be influenced by chronic gastritis - for example: gastroduodenal ulcer disease and stomach adenocarcinoma. H. pylori is concerned to be carcinogen of the first class. Calprotectin is a cytosol protein. It gradually releases from activated or lytic leucocytes. It is made of monocytes and neutrophiles. Calprotectin concentration in in faecal specimen correlates with the amount of leucocytes in intestine's lumen. This finding allows doctors to estimate the amount of intestine mucosas' infiltration by leucocytes according to evaluation of calprotectin levels. Calprotectin is highly sensitive to the presence of intestine inflammation, so it is a proper indicator of unspecific intestine inflammation diagnostics. The main aim of my bachelor thesis is to become familiar with identification methods of H. pylori and calprotectin in labs. Moreover, I aimed to become familiar with proper results interpretation of used methods. I estimated a hypothesis and an experimental question. In the theoretical part I focused on contemporary knowledge about H. pylori a calprotectin. I described common diagnostic methods, illnesses they cause and their treatment. The experimental part took place at Microbiology department of Stafila spol. sro laboratories. I examined the presence of H. pylori in faecal specimen by DIMA method. I determined the calprotectin levels by BUHLMANN method. I personally worked with 25 samples. I did my work in guidance of MUDr. Cihlová and her colleagues. I statistically examine my results. I made statistics of H. pylori frequency and determined the hypothesis. I also made statistic of negative, positive and strongly positive numerosity levels of calprotectin in faecal specimen and found the answer to my question.
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