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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
The interplay between StkP/PhpP phosphorylation pathway and diadenylate cyclase CdaA producing c-di-AMP in Streptococcus pneumoniae
 Beneš, Tomáš ; Buriánková, Karolina (advisor) ; Fišer, Radovan (referee)
The interplay between StkP/PhpP phosphorylation pathway and diadenylate cyclase CdaA producing c-di-AMP in Streptococcus pneumoniae Second messengers have emerged as a new field of bacterial reasearch. C-di-AMP is one of the most recently discovered second messengers. This compound is responsible for adaptation to multiple environmental stresses, especially those connected with cell wall damage. C-di-AMP is produced by enzymes called diadenylate cyclases. The most widespread diadenylate cyclase is CdaA. It was previously described that the activity of CdaA is regulated by phophoglocosamine mutase GlmM which participates in the synthesis of cell wall. GlmM is also phosphorylated by serine/threonine kinase StkP in Streptococcus pneumoniae. The aim of this thesis is to establish interplay between these three proteins with the emphasis on the activity of CdaA in S. pneumoniae. We have proved by two-hybrid system and co-immunoprecipitation that CdaA interacts directly with StkP. We also identified a weak phosphorylation of CdaA by StkP in vitro. We confirmed a decrease in CdaA activity in the presence of GlmM in coralyne reaction in vitro. On the other hand we did not detect any significant change of CdaA activity in the presence of StkP. Taken together we present a model which suggests a role of StkP in CdaA...
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Survey of bacterial nucleotide-based second messengers
 Beneš, Tomáš ; Branny, Pavel (advisor) ; Dolejšová, Tereza (referee)
Second messengers are small molecules that belong to one of the fundamental types of cell signalling. Their function is to transmit signals from extracellular or intracellular receptors to specific effector proteins. This type of signal transduction is evolutionarily ancient and conserved, occuring in every cellular organism. However, individual taxa differ in the specific compounds they use in signal transduction. In bacteria, different nucleotide derivatives are mostly used. The most important examples are cAMP, (p)ppGpp, c-di-GMP and c-di-AMP. Bacterial second messengers are involved in the regulation of metabolism, biofilm formation, stringent response, osmoregulation, protection against viral infection and many other processes. In addition to describing these signalling pathways, this work also deals with enzymes for synthesis and degradation of these small signalling molecules.
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