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Polymer systems for siRNA delivery
Blažková, Jana ; Pechar, Michal (advisor) ; Šťovíček, Vratislav (referee)
The process of RNA interference (RNAi) is a natural phenomenon posttranscriptionally controlling gene expression by means of small double-stranded RNA molecules (dsRNA). Small interfering RNA (siRNA) is a small dsRNA that can be used for targeted gene silencing as an alternative therapeutic treatment of genetic diseases. For in vivo administration, siRNA must be protected against degradation to ensure its efficient delivery to target cells using sophisticated vectors. This work is focused on description of non-viral vectors based on cationic polymers, forming polyelectrolyte complexes with siRNA (polyplexes), and surface-modifying hydrophilic polymers enabling protection of the vector during its transport in the bloodstream.
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Testování nových nanomateriálů pro teranostické aplikace u modelu myši
Donovalová, Alexandra
Theranostics is connecting two medical branches, diagnostic and therapy. It enables transport of drugs and diagnostic imaging in one step. Nanoparticles, nanotubes, lipozomes or apoferritin can be used as platform for targeted transport. Apoferritin (APO) is a protein from ferritin family, which is characterized by an empty cavity. Is possible encapsulate some cargo (e.g., drug) into this cavity. Encapsulation is done by pH change. There is a possibility to modify APO surface. It causes targeted transport to for example cancer cells. In this study, process of preparation of targeted nanotransporter based on APO is described; next, its characterization by in vitro experiments is shown. The prepared targeted nanotransporter delivered drug to cancer cells and in the same time was decreased toxicity on surrounding tissue and organs.
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Využití elektrochemických technik pro studium apoferritinu
Venusová, Eva
Theme of this diploma thesis is usage of electrochemical techniques for study of apoferritin, which is artificially synthetized protein derived from ferritin. Apoferritin has empty cavity which can be used for completion of broad amount of substances. Encapsulation of anticancer drugs can decrease their unwanted side effects such as strong toxicity and therefore increase it’s effectiveness at the tumor site. Substances chosen in this diploma thesis were standard platinum and platinum drugs cisplatin and it’s analogy oxaliplatin. Measurement was performed by electrochemical method differential pulse voltammetry by which was determined encapsulation capacity of apoferritin for chosen anticancer drugs. For determination size and zeta potential of nanoparticles was used colloid analyzator ZetaSizer Nano.
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Polymer systems for siRNA delivery
Blažková, Jana ; Pechar, Michal (advisor) ; Šťovíček, Vratislav (referee)
The process of RNA interference (RNAi) is a natural phenomenon posttranscriptionally controlling gene expression by means of small double-stranded RNA molecules (dsRNA). Small interfering RNA (siRNA) is a small dsRNA that can be used for targeted gene silencing as an alternative therapeutic treatment of genetic diseases. For in vivo administration, siRNA must be protected against degradation to ensure its efficient delivery to target cells using sophisticated vectors. This work is focused on description of non-viral vectors based on cationic polymers, forming polyelectrolyte complexes with siRNA (polyplexes), and surface-modifying hydrophilic polymers enabling protection of the vector during its transport in the bloodstream.
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