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Morphological differences in farm groups of salmonid fishes
Halačka, Karel
The aim of this study was examination of a influence species/line and stock conditions. This paper record first results form three groups of salmonid fishes – rainbow trout (from Italy and Denmark) and brook trout hybrides (S. fontinalis x S. alpinus). There were choosen morphological parameters observed – body contruction, weigth of intestine organs, epidermal and gill structure and erythrocyte size.
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Optimalization of photostimulation technology for delay sexual maturity of brook trout (Salvenilus fontinalis)
JUNG, Jakub
The aim of the present work, based on two experiments, was to evaluate the impact of photostimulation as a prevention for delaying the sexual maturation of brook trout (Salvenilus fontinalis). Another additional experiment was to describe which effect has photostimulation on the consequences of aggressive behaviour of males. Two experimental groups were established and differed in illumination sources: the metal halide lamps (HAL) and the light emitting diodes (LED). The photostimulation timing (photoperiod) was tested in following experiment. In both tested groups (HAL and LED) the photoperiod was prolonged to 18L:6D in comparison to the control group with natural photoperiod. The results shows that before spawning females and males in both tested groups (HAL and LED) reached significantly higher body weight up to 100 g (=0.05) due to desirable delayed sexual maturation (up to one month). By obtaining the same results in both groups, clearly profitable source of illumination was LED and the time for sufficient application before spawning was 67 days. Moreover photostimulation had also positively reflected in the elimination of aggressive behaviour of males. The results of the present study suggest that photostimulation may be applicable in the intensive culture systems of salmonids in the Czech Republic.
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The verification of the mass induction of triploidy in brook charr in operating conditions.
ŠVAGROVÁ, Kateřina
This bachelor thesis deals with the induction of triploidy in brook trout, Salvelinus fontinalis under farm conditions using a heat- and /or hydrostatic pressure shock. The first part involves the issues of polyploidy in fish, its induction, detection methods of polyploidy and its contribution to aquaculture. The second part deals with the technological process of induction of triploidy and subsequently, with evaluation of the effectiveness of both types of the shock upon ploidy level determine by flow cytometry. In this experiment, heat shock induced 80% of triploids in contrary to the hydrostatic pressure shock inducing triploidy in 100%. The pressure shock induced triploidy in brook trout more effectively than the heat shock and provided better operational results. Moreover, pressure shock displayed better fertilization of eggs, higher hatchability, and also higher percentage of triploids.
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Effect of alternative feed on content of omega 3 fatty acids in fish muscles
KREJSA, Jakub
The aim of my bachelor thesis was to evaluate the effect of fish oil substitution for rapeseed oil in the feed on the fat content and fatty acid composition in the flesh of Brook trout (Salvelinus fontinalis) in the finishing feeding technology. The experiment was conducted in 2012 for the period of 135 days. The individuals of Brook trout were divided into four groups. The first two groups were fed with the feed containing only rapeseed or fish oil. The feeding of the remaining two groups was divided into two phases. In the first phase the fish oil was partly replaced by the rapeseed oil. In the second phase the fish oil was replaced by the feed which contained fish oil only. It was proved that a half replacement of the fish oil with the rapeseed oil does not influence the content of fat in the flesh of Brook trout. We observed that the fish had the higher content of MUFA and n-6 PUFA and lower content of n-3 PUFA, DHA, EPA and n-3/n-6 with increasing period where the feed with rapeseed oil was served. Following application of the finishing feeding technology with the use of feed with fish oil had the opposite effects. Furthermore, the dilution model was proved to be highly reliable for the prediction of the fatty acids composition. Based on these findings, it is possible to predict changes of fatty acid composition in the flesh of Brook trout as well as to predict how long is needed to apply finishing feeding to reach required content of EPA+DHA or n-3 HUFA.
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