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Voltammetric Determination of 5-Nitroimidazole - The Structural Unit of Nitroimidazole Drugs
Šmídková, Monika ; Vyskočil, Vlastimil (advisor) ; Fischer, Jan (referee)
Presented Diploma Thesis deals with the study of electrochemical behavior of 5-nitroimidazole (5-NI), with the search for optimal conditions for its determination using techniques of DC voltammetry (DCV) and differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE) and at a boron-doped diamond film electrode (BDDFE), and with the comparison of reached limits of quantification (LQs). The optimal conditions found for measuring calibration dependences of 5-NI at the m-AgSAE in deionized water were as follows: Britton-Robinson buffer of pH 7.0 and 10.0 for DCV and DPV, respectively. At both thechniques, it is advisable to use an electrochemical regeneration of the electrode surface by the application of a couple of regeneration potentials Ereg,1 = 0 mV and Ereg,2 = −800 mV. The calibration dependences were measured in the concentration range from 1·10−6 to 1·10−4 mol·l−1 , with the LQs of 1.0·10−6 mol·l−1 for DCV at the m-AgSAE and 1.5·10−6 mol·l−1 for DPV at the m-AgSAE. The concentration dependences of 5-NI at the BDDFE were measured using the DCV technique in the BR buffer of pH 3.0. For the DPV technique, the optimal medium seemed to be the BR buffer of pH 2.0, however, because of the presence of an interfering peak in the supporting electrolyte,...
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Determination of benzophenone-3 on boron-doped diamond film electrode
Procházková, Kateřina ; Dejmková, Hana (advisor) ; Vyskočil, Vlastimil (referee)
Voltammetric behaviour of benzophenone-3 on a diamond film electrode doped by boron was studied by difference pulse voltammetry in an anodic area in the Britton-Robinson buffer medium. The influence of supporting electrolyte pH on the determination was monitored as well as the influence of methanol and acetonitrile content in the measured sample. pH 12 was selected as the optimum value for the determination. Methanol did not have any influence on the determination up to the content of 20 % (v/v), acetonitrile up to content of 70 % (v/v). A linear calibration dependence was measured in the medium of Britton-Robinson buffer pH 12 within the benzophenone-3 concentration range from 1·10-6 mol·L-1 to 1·10-4 mol·L-1 . Detection limit 1.5·10-6 mol·L-1 and determination limit 5.0·10-6 mol·L-1 have been reached. Key words Benzophenone-3 Boron-doped diamond film electrode Differential pulse voltammetry
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Voltammetric Determination of 5-Nitroimidazole - The Structural Unit of Nitroimidazole Drugs
Šmídková, Monika ; Vyskočil, Vlastimil (advisor) ; Fischer, Jan (referee)
Presented Diploma Thesis deals with the study of electrochemical behavior of 5-nitroimidazole (5-NI), with the search for optimal conditions for its determination using techniques of DC voltammetry (DCV) and differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE) and at a boron-doped diamond film electrode (BDDFE), and with the comparison of reached limits of quantification (LQs). The optimal conditions found for measuring calibration dependences of 5-NI at the m-AgSAE in deionized water were as follows: Britton-Robinson buffer of pH 7.0 and 10.0 for DCV and DPV, respectively. At both thechniques, it is advisable to use an electrochemical regeneration of the electrode surface by the application of a couple of regeneration potentials Ereg,1 = 0 mV and Ereg,2 = −800 mV. The calibration dependences were measured in the concentration range from 1·10−6 to 1·10−4 mol·l−1 , with the LQs of 1.0·10−6 mol·l−1 for DCV at the m-AgSAE and 1.5·10−6 mol·l−1 for DPV at the m-AgSAE. The concentration dependences of 5-NI at the BDDFE were measured using the DCV technique in the BR buffer of pH 3.0. For the DPV technique, the optimal medium seemed to be the BR buffer of pH 2.0, however, because of the presence of an interfering peak in the supporting electrolyte,...
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