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Vliv různých dávek a forem minerálních látek v dietě brojlerů na biochemický profil krve
Zającová, Karolína
This diploma thesis deals with study of influence of addition of different doses, forms of calcium and zinc into feed mixtures for broiler chickens. The aim was to find out how different doses or forms of these minerals affect the concentration of calcium and zinc in the blood of chickens. Ross 308 broiler chickens were used in the experiments. Calcium and zinc were fed to the feed mixture in the form of calcium carbonate, zinc oxide or organically bound zinc. The basal diet of the control group contained 2 g of calcium. Different doses of calcium carbonate (6 g, 9 g, 15 g / kg of feed mixture) were fed to three groups. Four groups were fed with the addition of zinc oxide at doses of 20 mg, 40 mg, 120 mg and 180 mg / kg of the feed mixture. In the next experiment, zinc was fed in various forms and different amounts to compare the effect of feeding organically bound zinc. At the end of the experiment, blood samples were taken during the slaughter and the concentrations of the studied elements were determined. Statistically significant differences in blood calcium levels have been demonstrated between the control group (containing Ca in the feed mixture 2 g / kg) and the groups of animals that we increased calcium (to 6 to 15 g / kg of compound feed). However, experimental groups did not have any significant differences. It has been found that increasing amounts of Ca in the feed have a negative effect on the concentration of magnesium in the blood. In the zinc subsidy experiment, we found conclusive differences between the control group and the experimental groups. At lower doses of Ca (less than 9 g Ca / kg) and Zn (less than 180 g Zn / kg) in the feed mixture, a proven correlation between the concentration of Ca and Zn in the feed and blood was demonstrated.
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Využití biochemického vyšetření krve v hodnocení výživy a metabolismu brojlerových kuřat
Šipošová, Aneta
This diploma thesis, which is based on the feeding experiment, deals with the influence of nutritional deficiency in broiler chickens nutrition on parameters of biochemical blood profile and subsequent determination of the dynamics of changes of specified blood parameters depending on the age of broiler chickens. The aim was to verify whether reducing nutrient concentration in the feed mixture has affected the biochemical blood profile and whether the biochemical parameters observed could be used as indicators of metabolic disorders arising from mistakes in chicken nutrition. For the experiment were used the hybrid broiler chickens Ross 308, which were divided into groups. Chickens of the control group were fed a standard granular feed mixture of BR2 and subsequently BR3 during the experiment. Chickens of the experimental group I were fed with a standard mixture of BR2 that was mixed with wheat (in ratio approximately 1:1) and mashed. In the second third of the experiment, was created experimental group II (a part of the chickens of the original test group I), where chickens were fed the same as the control group. The statistically significant difference (P ≤ 0.05) was among all groups in the assessment of weight of broiler chickens for the whole of the fattening depending on the age of chickens. Within the biochemical blood profile, resp. the evaluation of enzymes, macro elements, indicators of nitrogen and energy metabolism, some differences between groups were also statistically significant. However, the results of the experiment show that the determined biochemical parameters are not sensitive indicators of nutritional deficiency. Much more sensitive indicator of induced experimental nutrient deficiency was changes in live weight ofchickens and feed conversion ratio. The evaluation of the dynamics of changes inmonitored parameters depending on the age have been identified significant changes in some values. Increase in ALT = alanine aminotransferase, GGT = gama-glutamyltransferase, LD = lactate dehydrogenase, sodium, potassium, chloride, magnesium and total protein. Conversely, a significant decrease was found in ALP = alkaline phosphatase, uric acid and urea. Based on the experiment, we can say that biochemical blood profile is not sensitive indicator of the nutritional deficiency of broiler chickens. It is important to mention the effect of the age on the results because the number of parameters significantly changed during fattening.
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Porovnání zkrmování pšenic s různým obsahem flavonoidů a karotenoidů u laboratorních potkanů
Kovářová, Markéta
The effect of feeding wheat with various flavonoids and carotenoids was studied on rat blood parameters. The wheat RU 687-12 with purple pericarp, EF02-54/9 with blue aleuron and citrus wheat with yellow endosperm were used i the experiment. The wheat Novosibirskaya 67 was used as a control. Weight gains and feed consumption were monitored on 40 laboratory rats of the WISTAR strain. At the end of the experiment the rat blood was collected and biochemical parameters were determined in blood plasma. The rats fed with wheat from the Citrus group achieved significantly (P<0.05) highest increments whereas the lowest increments were achieved for rats fed with blue wheat mixture EF02-54/9. The biochemical parameters have shown significant differences between the groups (P <0.05). Significantly higher (P <0.05) values of AST, ALT, LD versus control were at the Citrus group and these values were in the range of the physiological limit. Rats from the Citrus and EF02-54/9 group had significantly lower (P <0.05) total protein values and higher albumin than the control group and the EF02-54/9 group. The Citrus and RU 687-12 rats groups showed a low content of the globulin fraction indicating a possible negative impact to the organism. The EF02-54/9 wheat-fed group had statistically lower (P <0.05) cholesterol content compared with the control group. In addition the blood parameters of rats did not show any potential negative effects to the liver.
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Vliv zkrmování barevné pšenice s modrým aleuronem na krevní parametry u laboratorních potkanů
Fajkusová, Martina
The experiment examined the effect of wheat with blue aleurone on blood parameters of laboratory rats the Wistar albino strain. In the experiment, 30 male rats were divided into 3 groups in 2 replicates. The first 4 groups were fed wheat varieties UC66049 and Skorpion. The last two groups received control wheat Bohemia. Consumption of feed, rat growths and feed conversion ratio were recorded during the experiment. After 72 days of testing, blood was collected. Blood plasma biochemistry profile was analysed. After a statistical evaluation, the finding was elevated the significant higher values of bilirubin and creatine kinase in the group fed Skorpion compared to UC66049 and control groups. The values of total protein were significantly lower in Skorpion wheat. Other evaluated parameters of the monitored blood plasma were non-significant.
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