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Active preparatives based on koji fermentation and medical mushrooms extracts
Živná, Kristýna ; Dzurická, Lucia (referee) ; Hoová, Julie (advisor)
This bachelor's thesis deals with the optimization of the growth of the rare species fungus of the hedgehog mushroom on cereal substrates for the purpose of the subsequent to cultivation of lactic acid bacteria. The theoretical part is focused on the description of the rare fungus species and the use of the cereals as substrate and the characterization of their active substances. It also deals with lactic fermentation and its use. In the experimental part, the preparation of the cereal substrate for the cultivation of the hedgehog mushroom was optimized. The method of inoculation of the cereals substrate with liquid mycelium in combination with plate mycelium was chosen as the most applicable. In this combination, the substrate does not dry out and grows evenly from both sides of the substrate. The composition of the cereal substrate with grown mycelium was characterized (antioxidant activity, carbohydrates, proteins). Finally, the substrate was inoculated with lactic bacteria. The lactic bacteria added nutritional value to the product. Fermentation products (antioxidant activity, carbohydrates, proteins) were characterized, and bacterial viability was analysed.
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