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Axon, in development and injury
Polčanová, Zuzana ; Kárová, Kristýna (advisor) ; Novák, Ondřej (referee)
The cytoskeletal structure of growth cones plays an important role in both the development of the nervous system and during periods of axon re- generation. The growth cone is a highly dynamic structure located at the tip of growing axons, providing navigation and movement. Signalling cascades are activated that lead to the regulation of the growth cone cytoskeleton, defining its displacement, rotation, or collapse. Despite advances in under- standing guidance cues and their mechanisms of action, knowledge of what happens to the nervous system after injury is lacking. Unlike axons in the peripheral nervous system (PNS), that are able to regenerate after neuronal injury, axons in the central nervous system (CNS) loose regenerative ability as they mature. Unravelling the mechanisms of axon guidance, together with their behaviour after axotomy and regeneration, is extremely important for the understanding of CNS injuries and to provide treatment of these injuries in the future.
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Pathophysiology of Spinal Cord Injury Studied by In Vivo Optical Imaging
Vančíková, Sabína ; Valášková, Barbora (advisor) ; Špicarová, Diana (referee)
Patients suffering from spinal cord injury experience physical, social, and vocational impairment. It is a condition often causing a permanent disability mainly due to axonal regeneration incapability in the central nervous system. The primary insult simultaneously damages cells in the lesion site and initiates a cascade of secondary cellular, vascular, and biochemical events extending the injury. These pathophysiological mechanisms are examined using multiple approaches. Novel imaging techniques complement classical histopathological methods and neuroanatomical tracing. Recent studies employ transgenic mice and two-photon microscopy to observe single cells in the injury site and the nearby vasculature in vivo longitudinally. In vivo optical imaging enables studying of axonal responses, such as degeneration, regeneration, and neurovascular interactions. It also gives an opportunity to assess the effects of applied drugs directly. New findings lead to a better understanding of the pathophysiology of spinal cord injury, resulting in the ability to develop other therapeutic strategies improving the outcome after injury. Keywords: spinal cord injury, pathophysiological mechanisms, axonal regeneration, Wallerian degeneration, animal models, transgenic mice, in vivo imaging, two-photon excitation microscopy
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