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Effect of atorvastatin on endoglin expression and function in hyperlipidemia-induced endothelial dysfunction
Holadová, Adéla ; Tripská, Katarína (advisor) ; Fikrová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of atorvastatin on endoglin expression and function in hyperlipidemia-induced endothelial dysfunction Author: Adéla Holadová Supervisor of Diploma Thesis: PharmDr. Katarína Tripská, Ph.D. Aim: The aim of this thesis was to determine how atorvastatin affects the expression and function of endoglin and related biomarkers of inflammation/endothelial dysfunction at different concentrations and times in human endothelial cells from the aorta, in which hyperlipidemia was induced by using palmitic acid. Methods: In the diploma thesis, we worked with human aortic endothelial cells. We premedicated them with atorvastatin/palmitic acid at different times and concentrations. Using flow cytometry, we measured the protein expression of endoglin. Furthermore, we used the qRT-PCR method to determine the level of mRNA expression of endoglin, its transcription factors and biomarkers of inflammation. Using the ELISA method, we detected the levels of soluble endoglin. Results: We demonstrated that atorvastatin at a concentration of 5μM after 16 hours of premedication significantly increased the mRNA expression of endothelial nitric oxide synthase, and conversely decreased the...
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Fotochemická degradace hypolipidemika atorvastatinu s využitím heterogenní fotokatalýzy.
VONEŠOVÁ, Adéla
The Bachelor s thesis is focused on hypolipidemic drugs statins and their representative atorvastatin. In the theoretical part of the thesis, the function of statins is introduced, studies about statins toxicity for freshwater organisms are presented, fundamentals of heterogeneous photocatalysis are explained and examples of usage of the method for pollutants degradation are given. Experimental part presents kinetics of atorvastatin degradation on TiO2 photocatalyst and a test of toxicity of atorvastatin photoproducts on a water plant (Lemna minor).
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Statiny - jejich použití, mechanismus účinku, vedlejší efekty. Atorvastatin a možnosti jeho fotochemické degradace v přírodních vodách.
PETRÁŇOVÁ, Pavla
The Bachelor´s thesis deals with a group of hypolipidemic drugs, statins. In the theoretical part, their development, characteristics, mechanism of action and side effects are presented. The experimental part is focused on one representant of statins, atorvastatin, its possible photochemical degradation in aqueous solution under light conditions relevant to natural waters, and a potential toxic effect of atorvastatin itself and of the mixture of products of atorvastatin photochemical degradation on a water plant Lemna minor.
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