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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Infection pathway of Hymenoscyphus fraxineus and its interactions with ash mycobiota
 Haňáčková, Zuzana ; Koukol, Ondřej (advisor) ; Adamčíková, Katarína (referee) ; Botella Sánchez, Leticia (referee)
Ash dieback is a disease that affected populations of native ash species throughout Europe at the beginning of the 21st century. It causes necrotic spots on the leaves, necrosis of shoots and branches and, especially in young individuals, the total death of the tree. In particular, F. excelsior is attacked by ash dieback. The causative agent of the disease is the invasive ascomycete fungus from Asia - Hymenoscyphus fraxineus. The aims of this dissertation thesis were as follows: 1) to study population structure of H. fraxineus at different scales - at regional scale (Czech populations) and at the level of particular ash petioles, this latter was to serve as a confirmation of the pathogen infection pathway; 2) to elucidate the effect of H. fraxineus on saprotrophs in the litter (namely H. albidus) and 3) to compare endophytic mycobiota of tolerant and susceptible F. excelsior trees and to test antagonistic interactions of these fungi against the pathogen. Analysis of Czech populations showed lower average gene diversity compared to other European populations with one Czech population differing significantly from the rest due to the lowest allelic richness and very low average gene diversity. The results indicate that despite minimal differences among European populations, local ecological or...
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Vertical gradient of Hymenoscyphus fraxineus spore dispersal at infected locality in Boršov nad Vltavou
 ŠTOIDL, Petr
Hymenoscyphus fraxineus is a pathogenic sac fungus causing ash tree necrosis throughout Europe, spreading predominantly through the air using ascospores. The aim of this thesis was to quantitatively describe the occurrence of spores of the causative agent of ash trees necrosis at different heights above the source of infection. The results were subsequently used to consider the inoculum potential of this pathogenic fungus for longer distance spreading using air currents. The qPCR method was used for the detection and quantification of DNA concentration. Based on a statistical analysis of the obtained molecular data a dependence of the occurrence of different amounts of H. fraxineus on height was proved. The main contribution of this thesis lies in charting the range of the H. fraxineus pathogen spread in the air column which is directly related to the range of spores to greater distances.
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