|
|
Role cytokininů v regulaci vyrůstání pupenů hrachu
Vetter, Martin
The diploma thesis was aimed to explain the role of cytokinins in regulation of cotyledonary bud outgrowth in pea (Pisum sativum L.). Decapitation of the apex and subsequent application of IAA lanolin paste containing also BAP caused increased expression of genes PsDRM1 and PsBRC1 associated with dormancy. It suggests that cytokinins may have effect on the amount of transported auxin in the major stem. Application of cytokinin to the cotyledonary buds of intact plants activated their outgrowth. Imunolocalization showed polarization of PIN1 proteins in the treated buds, on contrary to untreated buds. It means that cytokinin directly influenced polarization of PIN1 proteins and the subsequent canalization of the polar auxin transport.
|
|
|
Expresní profily vybraných genů při vyrůstání kotylárních pupenů hrachu
Psotová, Martina
The diploma thesis was aimed to monitor the expression profiles of selected genes in the growing of cotyledonary buds in pea (Pisum sativum L.). The apical stem was decapitated and 0.5% auxin paste was applied to the stump. From the treated plants cotyledonary buds were collected in intervals 0, 1, 3, 6, 12, 24, 48 and 120 hours. The biological material was analyzed for expression of genes PsDRM1, PsBRC1, PsAUX1, PsPIN1, PsYUC1. A gene expression normalization factor was calculated based on constitutive genes PsEF1-alpha, PsActin and Ps beta-tubulin. Application of auxin to the stem stump prevented the growth of cotyledonary buds. This inhibition of cotyledonary buds was accompanied by increased expression of dormancy markers PsDRM1, PsBRC1 and auxin biosynthesis gene PsYUC1 and decreased expression of the auxin transporter genes PsPIN1 and PsAUX1.
|
|
|
Regulácia vyrastania kotylárnych pupeňov hrachu
Kucsera, Attila
This work was aimed to achieve more details in regulation of cotyledonary bud outgrowth of pea plants (Pisum sativum L.). Plants were studied after decapitation of the apex and removal of cotyledons and their subsequent substitution with agar gels containing different susbstances. Experiments were perform in light and dark conditions. The obtained results proved that auxin could regulate outgrowth of pea cotyledonary buds. Furthermore, sucrose showed partly inhibitory effect on bud outgrowth. This inhibitory effect was pronounced if plants were cultivated in dark. Next experiments showed that cotyledons could regulate apical dominance depending on light or dark conditions.
|
|
|
Vplyv fotoperiódy na apikálnu dominanciu u hrachu
Füleky, Martin
The part of my thesis was to make literary research about influence of photoperiod, light intensity, phytohormones (mainly auxins, cytokinins and strigolactones) and other factors on shoot branching in pea. The experiment was focused on finding the influence of different day lengths (8 hours, 16 hours, 24 hours) on shoot branching in pea. In experiment intact plants, plants decapitated above 3rd node and plants decapitated above cotyledons were analyzed. Increases in length of the branches were observed for 5 days. Graphs and statistical analysis were generated from the data. After that, samples from plants were taken. These samples were used in semiquantitave RT-PCR with analysis of intensity of gene expression from agarose gel.
|
|
|
Růstové korelace u rostlin konopí setého
Michálková, Klára
The teoretical part of my bachelor thesis Growth correlations in cannabis plant is mainly focused on the issue of growth correlations. In the bachelor thesis, concrete examples of growth correlations are characterized including more details on problematics of apical dominance of the stem. The basic botanical, morphological and genetic characteristics of cannabis are also described, including important content substances. In the experimental part, the intensity of shoot branching of intact plants of selected technical and medical cannabis genotypes is investigated. The increase in length of axillary buds of first, second and third node was measured. Within the period of a few days, plant response to decapitation and exogenous auxin application was tested. In technical cannabis there was a strong response to exogenous application of auxin. On the other hand in the most cases of medical cannabis there was a reduced sensitivity to exogenous application of auxin. On the topic of shoot branching it was observed that the branching of medical hemp genotypes is significantly more intensive compared to the varieties od technical cannabis. The results of the grafting experiment then imply that the rootstock of technical cannabis did not influence the intensity of shoot branching of the medical cannabis scion.
|
|
|
Využitie muntantov ramosus pri štúdiu fytohormonálnej regulácie vetvenia stonky u hrachu
Polko, Peter
This thesis deals with effect of phytohormonal application on apical dominance, stem anatomy and expression of PsPIN1 in garden pea (Pisum sativum) variety Paloma and from this variety derived mutant rms4. In this thesis effect of decapitation, exogenous application of auxin (IAA, in a form of lanoline paste) and strigolactone (GR24, in a form of admixture in nutrient solution) on growth activity of axillary buds from nodes 1st to 4th was studied. After decapitation (above 4th node) "wild-type" plants reacted by increased intensity of axillary buds growth in almost all observed nodes and application of IAA inhibited growth of axillary buds to level of intact plants. In rms4 plants response on decapitation was only observed in axillary buds of the 4th node along with there was observation of insensivity to IAA in other observed nodes. GR24 inhibited growth of axillary buds in a small extent in "wild-type" plants and in rms4 mutants there was no observation of this inhibitory effect of strigolactone. Furthermore this thesis deals with effect of decapitation on [3H] - IAA transport from axillary bud in to young plants stems. Polar auxin transport capacity in stem is slightly higher in rms4 mutant and after decapitation axillary buds exported more auxin compared to "wild-type" plants. De novo vascular differentiation was observed 5 days after application of IAA in stem of both genotypes. Expression of PsPIN1 increased after IAA treatment both in Paloma and in rms4 mutant. This increase was higher in rms4 mutant compared to variety Paloma.
|
|
|
Vliv transportu auxinu na regulaci vyrůstání kotylárních pupenů hrachu
Macoszek, Ondřej
The diploma thesis was aimed on role of polar auxin transport in regulation of cotyledonary bud outgrowth in pea plants (Pisum sativum L.) in relation to competitive canalization of auxin sources. Decapitation of growth apex, as primary source of auxin, causes release of cotyledonary buds from inhibition. This was followed by increased expression of genes for auxin transport proteins PsPIN1 and PsAUX1 and decrease in PsDRM1 and PsBR1 gene expression that are related to dormant state of plant tissues. In the next phase, different types of exogenous auxin application on decapitated stumps and cotyledonary bud growth dynamic were carried out. Auxin application on decapiteded stump caused inhibition of cotyledonary bud growth, its application on right or left side of the stump inhibits cotyledonary bud on the side of application. If auxin on the both sides of stump was applied, cotyledonary bud growth was not inhibited, on contrary to their separation with a plastic sheet, which resulted in significant growth inhibition of both buds. In case of both-sided application, these sources of auxin prevents each other in canalization. If these two sources of auxin were separated with a plastic sheet, canalization of auxin flow was observed and two independent auxin channels were formed. Analogy of this competition can be expected in the case of cotyledonary buds, as two potential auxin sources.
|
|
|
Role RMS2 v regulaci apikální dominance
Hiršová, Veronika
The role of RMS2 in regulation of apical dominance was studied with the use of rms2 mutants and wild type cv. Torsdag. Phenotypic changes and changes in polar auxin transport (by imunnolocalization of PIN1) in rms2 mutants and after auxin application were observed. The phenotypic changes of rms2 mutants against cv. Torsdag are enhanced branching from the first two nodes but much less than branching of strigolactone deficient mutants. Auxin application both on intact and decapitated rms2 mutants led to enhancement of growth of the buds adjacent to the place of application in contrary to wild type in that the application inhibits the growth of adjacent buds. Auxin application to the cut surface of decapitated stem or from the side of the stem led in the primary application always to canalization. Secondary application 24 hours later led to canalization only in rms2. Originally proposed feedback regulation of RMS1 expression by RMS2 was changed to RMS2 as being a negative regulator of polar auxin transport.
|
|
| |
|
| |
guest ::
