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Stanovení aflatoxinu ve vybraných výrobcích
Musilová, Martina
The diploma thesis "Determination of aflatoxin in selected products" deals mainly with aflatoxin, its occurrence in raw materials of plant origin and methods of decontamination. The studied toxin is produced by fibrous micromycetes primarily from genus Aspergillus is their secondary metabolites. It is very widespread and is a frequent cause of retention of raw materials at the border. Factors that affect production of aflatoxin are, among other things, water activity and pH and are also determined in samples and mentioned in theoretical part. A total of 30 samples were tested for aflatoxin B1, including batch of apricot jam, two batches of Svatojánské ořechy and two batches of Jarní hustopečské mandle. A heterogeneous competitive enzyme immunoassay (ELISA) was used for the analysis. Detectable amounts of AFB1 in the range of 1,26 - 1,66 μg∙kg-1 showed 26,67 % of samples. These values were determined in samples of Svatojánské ořechy and Jarní hustopečské mandle, the apricot jam did not contain measurable amount of AFB1.
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Influence of fungi and their metabolites on the health status of wildgame.
BOHÁČKOVÁ, Lenka
The aim of this thesis was to evaluate the examined samples of liver tissue to find out the amount of aflatoxin in deer game. A total of 221 samples were examined for analysis purposes using the RIA method. Samples were obtained from dead and captured heads of deer game coming from private game reserves and game reserves belonging to the Forests of the Czech Republic. Samples of deer game were divided into groups according to their types, sex, and age. Sex was determined visually, and age via the development and grinding of the teeth. The highest concentration of B1 aflatoxin in liver was measured in a 6 year-old dead female (7,39 g.kg-1). According to the statistical evaluation (P <0,05), a significant difference was proven between dead and captured heads, between males and females, and even between age groups. There is a higher aflatoxin B1 concentration in dead heads´ liver, approximately in 2,12 g.kg-1 higher than in captured heads. Females´ liver proved to contain a higher concentration of B1 aflatoxin, approximately in 0,63 g.kg-1 higher than in males. Age groups have proven that the older the animals, the higher the B1 aflatoxin concentration in their liver.
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Hodnocení kvality přikrmování spárkaté zvěře na základě obsahu aflatoxinů
Kohoutová, Petra
This diploma thesis deals with the problem of aflatoxin in selected organs of fallow game, roe deer and for comparation of petty game with hare. The aim was to evaluate the free and preserve breeds at a sufficient amount of samples and to assess the suitability of a feeding.There were assessed the parenchymal tissues and kidneys and liver. It evaluated 176 collected biological tissues, including 118 from free places and 58 samples from specialized preserve.After the screening method RIA complemented by gas chromatography, were evaluated concentrations of aflatoxin B1. Diferences of feeding, which were assessed on based of results of evaluation in preserves.Subsequently the problem of submitting feeds was solved, and it was made a manual for submission of a feed.
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