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Factors influencing cell adhesion and the formation of yeast biofilms on solid surfaces
Světlíková, Daniela ; Palková, Zdena (advisor) ; Groušl, Tomáš (referee)
Yeasts are unicellular microorganisms that naturally aggregate to form multicellular structures. One of these formations is biofilm. The key properties of biofilm in S. cerevisiae are determined by the expression of the FLO11 gene. The large FLO11 gene promoter is the target of several pathways and factors, resulting in the complex regulation of this gene. Among the regulators of the FLO11 gene are the conserved Cyc8p and Tup1p proteins, which play a role in the regulation of many processes, especially as a corepressor complex. In previous years, our laboratory has demonstrated an antagonistic effect of the Cyc8p a Tup1p regulators on adhesion of BRF strain (Nguyen et al., 2018). In my work, I investigated whether this regulation is also valid in the selected clinical isolate S. cerevisiae YJM320 and whether, as in the BRF strain, positive regulation of FLO11 via Tup1 and negative regulation via Cyc8 exist here or whether their effect is different in this strain. The broader goal of my work was to identify the role of various factors on adhesion as the first step in biofilm formation. My work began by testing the effect of nutrients and temperature on the adhesion of strains BY, BRS and BRF and continued by testing the adhesion of selected clinical isolates of Saccharomyces cerevisiae and Candida...
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Development and differentiation of different types of yeast colonies: Regulation of metabolic diversification and development of cells with novel properties
Maršíková, Jana
Yeasts are unicellular organisms, but on a solid substrate they are capable of forming complex organized structures that behave like primitive multicellular organisms. Examples of these structures include colonies and biofilms, whose cells interact with each other, coordinate their growth and development, differentiate spatially and form specialized cell subpopulations in which specific processes and regulatory pathways occur. The basis of cellular differentiation and specialization is the formation of gradients of nutrients, metabolites and signaling molecules. Thus, multicellular yeast communities differ significantly from planktonic populations in their characteristics. The aim of this work is to increase knowledge related to the development and differentiation of both smooth and structured colonies of the yeast Saccharomyces cerevisiae. The literature introduction of the thesis provides an overview of the current knowledge on the development of yeast colonies and biofilms, especially of S. cerevisiae species, and also includes selected regulations important for the formation of multicellular populations. The thesis provides insights into the antagonistic function of the transcriptional regulators Cyc8p and Tup1p in the development of structured biofilm colonies. Genome-wide transcriptomic...
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Development and differentiation of different types of yeast colonies: Regulation of metabolic diversification and development of cells with novel properties
Maršíková, Jana ; Palková, Zdena (advisor) ; Heidingsfeld, Olga (referee) ; Demnerová, Kateřina (referee)
Yeasts are unicellular organisms, but on a solid substrate they are capable of forming complex organized structures that behave like primitive multicellular organisms. Examples of these structures include colonies and biofilms, whose cells interact with each other, coordinate their growth and development, differentiate spatially and form specialized cell subpopulations in which specific processes and regulatory pathways occur. The basis of cellular differentiation and specialization is the formation of gradients of nutrients, metabolites and signaling molecules. Thus, multicellular yeast communities differ significantly from planktonic populations in their characteristics. The aim of this work is to increase knowledge related to the development and differentiation of both smooth and structured colonies of the yeast Saccharomyces cerevisiae. The literature introduction of the thesis provides an overview of the current knowledge on the development of yeast colonies and biofilms, especially of S. cerevisiae species, and also includes selected regulations important for the formation of multicellular populations. The thesis provides insights into the antagonistic function of the transcriptional regulators Cyc8p and Tup1p in the development of structured biofilm colonies. Genome-wide transcriptomic...
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