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Toxicity determination of polyfluorinated and perfluorinated organic substances
Otrubová, Lucie ; Čabala, Radomír (advisor) ; Rucki, Marian (referee)
The goal of the bachelor thesis was the determination of toxicity of fluorotelomer alcohols. The toxicity was measured as ET50 by the oligochaete Tubifex tubifex. This test belongs to group of alternative methods which are faster and cheaper but they provide reliable data as well as the presently used classical methods. ET50 is the exposure time when 50 % of Tubifex tubifex individuals stop moving. EC50 of magnese chloride dihydrate was measured for the control of its correctness of determination and its acute toxicity indice is equal to log (EC50) = -0,845 ± 0,033 [log (mmol/l)]. EC50 is the concentration of the tested substance when 50 % of Tubifex tubifex individuals stop moving. 1H,1H,2H,2H-perfluorooctan-1-ol and 1H,1H,2H,2H-perfluorodecan-1-ol were the tested substances. Destilled water and solution of dimethyl sulfoxide in water (5 %) were used to prepare the saturated solutions of the tested substances. The conncentrations of the saturated solutions were determined on GC-MS by absolute calibration method. The value of log (EC50) for dimethyl sulfoxide solution was measured to determine how it influences Tubifex tubifex during exposure time of 2,5 hours. KEY WORDS: fluorotelomer alcohols, alternative method, Tubifex tubifex
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