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Function analysis of overexpression of gene for a Myb transcription factor from tomato, potential target of viroid infection
ONDRUŠ, Jaroslav
The main aim of this thesis was to find out whether gene Trifoliate, encoding a transcription factor from Myb family could be target of viroid PSTVd and is involved in its unknown interactions with host plant molecules, responsible for PSTVd pathogenesis. For this purpose, transformation of wild type plants Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, and mutant lof-1 Arabidopsis thaliana with Trifoliate was made, changed anatomy and morphogenesis of transformants has been observed. Due to our research and already known facts we suggest Trifoliate has a great importance in regulation of specific organs development. Manipulation with expression of this gene in some cases leaded to triggering similar sympthoms as PSTVd causes.

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