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Computational simulation of mechanical behaviour of living cell
 Wojtek, Lukáš ; Fuis, Vladimír (referee) ; Jadrná, Lucie (advisor)
The presented thesis deals with the structure of animal cells and their mechanical response to mechanical loading. Mechanically important component in the creation of a computational model of a cell is cytoskeleton. It consists of actin filaments, intermediate filaments and microtubules. The work focuses on determining the stiffness of microtubules and its effect on the overall stiffness of cells. Due to the different properties of microtubules in tension and compression, an effective modulus of elasticity has to be found using computational modeling. The influence of the newly found modulus of elasticity on the overall mechanical response of the cells was ensured by a simulation of a mechanical test of an adherent cell under pressure. That was achieved by using a hybrid computational model of cells containing, in addition to the cytoskeleton, the continuum of cells – nucleus, cytoplasm and cytoplasmic membrane. The results of this thesis should serve as a basis for further research on the effect of cytoskeleton stiffness on the mechanical response of the cell.
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