|
|
Photoluminiscence study of thin layers of MoS2
Kuba, Jakub ; Kunc,, Jan (referee) ; Bartoš, Miroslav (advisor)
The thesis deals with study of thin layers of transition metal dichalcogenides, especially of molybdenum disulfide. Nanostructures were fabricated on two-dimensional crystals of MoS2 and WSe2. Within followed analysis attention was paid to the photoluminescence properties. In the thesis transition metal dichalcogenides are reviewed and description of the modified process of preparation by micromechanical exfoliation is given.
|
|
|
Designing novel catalyst loaded electrode materials towards electrochemical sensing to energy applications
Kandambath Padinjareveetil, Akshay Kumar ; Escarpa, Alberto (referee) ; Vidal, Salvador Pané (referee) ; Pumera, Martin (advisor)
Exponenciální nárůst potřeby energie spolu s rostoucími nároky na zdravotnický sektor způsobuje velká rizika pro přežití lidstva na Zemi. V současné době probíhá intenzivní výzkum s cílem nalézt ideální řešení k překonání těchto globálních výzev. Využití elektrochemických energetických technologií je velmi žádané pro zmírnění rostoucích energetických požadavků ve spojení s výrobou novějších elektrokatalyzátorů, které jsou levné, účinné a odolné pro využití takových zařízení pro komerční aplikace ve velkém měřítku. Ačkoli se opakovaně studuje několik katalytických materiálů, existuje potřeba dalšího výzkumu katalyzátorů. V této studii jsou představeny nové techniky přípravy katalyzátorů pro vývoj aplikací těchto katalyzátorů pro výrobu vodíku a amoniaku. S rozšiřujícím se rozsahem technologie 3D tisku je také podrobně diskutována výroba elektrokatalyzátorů prostřednictvím této technologie s důrazem na elektrokatalytické aplikace, jako je výroba vodíku, syntéza amoniaku a redukce oxidu uhličitého. Kromě katalýzy tato práce také hodnotí potenciální možnosti využití technologie 3D tisku pro biomedicínské aplikace, jako jsou elektrochemické sensory. V sourhnu, práce si klade za cíl výzkum pokročilých elektrokatalůyzátorů spolu s poskytnutím vhledu do navrhování a konstrukci materiálů aktivních elektrod pro přeměnu energie a aplikace elektrochemických sensorů.
|
|
|
Identification and Characterization of Genetic Aberrations in Acute Childhood Leukemia
Lukeš, Julius ; Kubričanová Žaliová, Markéta (advisor) ; Machová Poláková, Kateřina (referee) ; Živný, Jan (referee)
Childhood acute leukemias are genetically complex disorders, with recurrent or random aberrations found in most patients. Their proper functional characterization is crucial for understanding the role they play in the process of leukemogenesis. We aimed to identify and characterize the genetic background of two leukemic entities. The transient myeloproliferative disorder (TMD) is a preleukemic condition that occurs in 10% of newborns with Down syndrome. Trisomy 21 together with in-utero gained mutations in the GATA1 gene are essential in TMD and represent an ideal "multi-hit" model to study leukemogenesis. We investigated an alternative pathogenic mechanism enabling TMD development in a confirmed absence of trisomy 21. Novel deletions in the GATA1 and JAK1 genes were described as potential drivers of this TMD. The deletion D65_C228 in GATA1 results in the expression of an aberrant isoform, which is predicted to lose transactivation potential and, more importantly, to partially lose the ability of recognizing physiological DNA binding sites, possibly triggering TMD alone. Our thorough characterization of JAK1 F636del questions its role in TMD development. Analysis of JAK/STAT signaling suggested decrease of kinase activity upon F636 loss. Cells harboring the aberrant JAK1 did not obtain cytokine-...
|
|
|
Identification and Characterization of Genetic Aberrations in Acute Childhood Leukemia
Lukeš, Julius ; Kubričanová Žaliová, Markéta (advisor) ; Machová Poláková, Kateřina (referee) ; Živný, Jan (referee)
Childhood acute leukemias are genetically complex disorders, with recurrent or random aberrations found in most patients. Their proper functional characterization is crucial for understanding the role they play in the process of leukemogenesis. We aimed to identify and characterize the genetic background of two leukemic entities. The transient myeloproliferative disorder (TMD) is a preleukemic condition that occurs in 10% of newborns with Down syndrome. Trisomy 21 together with in-utero gained mutations in the GATA1 gene are essential in TMD and represent an ideal "multi-hit" model to study leukemogenesis. We investigated an alternative pathogenic mechanism enabling TMD development in a confirmed absence of trisomy 21. Novel deletions in the GATA1 and JAK1 genes were described as potential drivers of this TMD. The deletion D65_C228 in GATA1 results in the expression of an aberrant isoform, which is predicted to lose transactivation potential and, more importantly, to partially lose the ability of recognizing physiological DNA binding sites, possibly triggering TMD alone. Our thorough characterization of JAK1 F636del questions its role in TMD development. Analysis of JAK/STAT signaling suggested decrease of kinase activity upon F636 loss. Cells harboring the aberrant JAK1 did not obtain cytokine-...
|
|
|
Photoluminiscence study of thin layers of MoS2
Kuba, Jakub ; Kunc,, Jan (referee) ; Bartoš, Miroslav (advisor)
The thesis deals with study of thin layers of transition metal dichalcogenides, especially of molybdenum disulfide. Nanostructures were fabricated on two-dimensional crystals of MoS2 and WSe2. Within followed analysis attention was paid to the photoluminescence properties. In the thesis transition metal dichalcogenides are reviewed and description of the modified process of preparation by micromechanical exfoliation is given.
|
|
|
Physiotherapy of a displaced disc with reposition of a temporomandibular joint
BROŽKOVÁ, Aya
The topic of my bachelor thesis is physiotherapy of a displaced disc with reposition of a temporomandibular joint. It is a functional disorder, when the mandible disc is not in a physiological position and creates an obstacle for a condyle movement. When mouth is fully opened the disc does not return to its original position. This moment is characteristic with a clicking sound. Another symptom is inclining of the chin to the disordered site when the mouth is being opened. Main cause of TMD is stress, bad occlusion and a long-term micro trauma of TMJ. It is a topical issue, because an increase of stress in daily life causes also more TMD. Almost 70 % of the population has the TMD, in most of the cases the TMJ disc is in a wrong position. The therapy of this kind of disorder should be done by physiotherapist. Unfortunately knowledge of diagnosis and healing of the TMD is not sufficient. The disorder is recognized usually by dentists, who send a patient to a specialized clinic. The goal of my work was examining of mastication and neck muscle spasms. In the work there are also described physiotherapeutic possibilities of healing including postural strategy. I have created informational booklet for patients, in which saving method for TMJ healing, exercises, ergonomic principles and correct posture both when sitting and standing are presented. The research was done by 14 case analysis, analytic-inductive method and experimental work. Patients were randomly divided into three groups. As a first step TMJ and cervical spine of each patient and their posture were examined. The examination of TMJ consisted of diagnosis, extent and symmetry of an opening movement and palpation examination of the cervical spine. Therapy was six weeks long. Each patient was introduced to TMJ saving method, which is based on limitation of a TMJ movement, eating soft food and elimination of stress. In the first group (5 patients) the therapy was focused only on mastication muscles. Patients were supposed to be exercising to loosen the muscles of mastication. Exercises were chosen after palpation examination. Third group (4 patients) did neck muscle exercises and self massage. Second group (5 patients) did the therapy of both first and third group. Results of the therapy show, that there is a possible connection between TMD and cervical spine dysfunction. There were positive (evaluated subjectively) changes in both TMJ and in all groups. The condition of TMJ is influenced by neck muscle therapy and the condition of cervical spine is influenced by mastication muscle therapy. Best results had the second group. All patients but one improved (one lady had a TMJ inflammation). There were no deterioration of TMJ and cervical spine condition either. There were some worsening in group one and three. The best therapy is a combination of exercises of both mastication and neck muscles. I would like to point out a fact that TMJ is one of the most complicated and busy joints in human body. Both TMJ are connected to mandible so dysfunction of one joint can lead to problems with the other one. Physiotherapy is needed most in cases of functional disorder.
|
guest ::
