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Cytochemická vyšetření akvitivity nukleolů po podání genisteinu na modelovém objektu
PICMONOVÁ, Veronika
This thesis deals with exploration of the effect of genistein on proteosynthetic activity of nucleoli by observing changes in their morphology. The effect of genistein on the function of nucleoli was observed in hemocytes of the experimental animal Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). 4 types of hemocytes were found in the hemolymph of S. littoralis (Boisduval): granulocytes, prohemocytes, plasmatocytes and spherulocytes. The effect of genistein on individual types and concentration of hemocytes is not clear. Genistein was applied in doses of 0,135 mg and 0,54 mg per larva, and subsequently changes in morphology, number and size of nucleoli were examined upon 8 and 24 hours. Furthermore, changes in morphology of the hemocytes were examined, as well as their concentration using toluidine blue staining, silver staining, panoptic staining and using the Türk{\crq}s solution. For the purpose of changes assessment, the material was processed using an optical microscope and the computer software Nis Element AR 2.30. In the course of observation, changes in the number of nucleoli were found upon application of genistein. The number of compact nucleoli and micronucleoli increased. On the contrary, the number of ring-shaped nucleoli decreased. The area of nucleoli increased upon application of lower dosage of genistein (0,135 mg); however, upon action of the higher dose (0,54 mg), it decreased, on the contrary. Nucleolar index values remained without rather significant changes. In the biomodel used, genistein evoked changes which can be viewed as immune responses from the viewpoint of hematotoxicology.
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Effect of recombinant toxin Cry3Aa on development of \kur{Spodoptera littoralis}
HUSÁKOVÁ, Jana
This bachelor thesis compares the effect of recombinant and natural Bacillus thuringiensis Cry3Aa toxin upon the development of non-target insect Spodoptera littoralis (Lepidoptera, Noctuidae). Caterpillars of Spodoptera littoralis were fed with Premix artificial diet containing both toxins in 8 ppm concentration. Following characteristics were monitored: weight of larval increments, pupal weight, duration of the 5th, the 6th, prepupal and pupal instar, number of laid eggs and number of hatched larvae. The efficiency of conversion of ingested food (ECI) was calculated and growth curves were constructed. No significant effect of both toxins was recorded.
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