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Zhodnocení kvality ejakulátu vybraných plemen psů
Hronková, Petra
The theoretical part of the thesis on "Evaluation of semen quality selected breeds of dogs", provides a brief summary of available information obtained from literature dealing with dogs reproduction, anatomy male genital tract of the dog and basic laboratory techniques of animal semen analyzes. Practical work was focused on evaluating quantitative and qualitative parameters of ejaculate different breeds of dogs from the kennel Bohemia Alké. Was investigated primarily affect the order of collection, ages, body frame, time of day and length of the sampling collection itself on ejaculate volume, activity concentration, total sperm count and percentage of occurrence patomorfologických defects. Subscriptions ejaculate, macroscopic and microscopic evaluation was conducted in field conditions in the home where the dogs lived. To evaluate the percentage of damaged sperm pathomorphological were made morphological coatings, which were transported to the laboratory department reproduction of farm animals and the Institute of Animal Breeding, Faculty of Agronomy, Mendel University in Brno.
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Cryopreservation of common carp (\kur{Cyprinus carpio} L.) sperm under different freezing conditions
SOCHOROVÁ, Denisa
In the present study, we examined several cryoextenders previously used by several authors and various freezing protocols to determine the relative importance of each parameter on sperm freezing. The effects of controlled seeding and changes in cooling rate at different stages of freezing were also examined. Sperm samples from seven individual carp males were frozen in 0.5 ml straws by conventional freezing. Cooling rates were determined by monitoring the sample's internal temperature. We compared four freezing protocols, which involved placing sperm samples at various levels (1, 3, 6, and 9 cm) above the liquid nitrogen (LN) surface (corresponding to -190, -150, -110, and -70 °C, respectively) for 20 min followed by transferring the samples into LN. Freezing at 3 cm above the LN surface resulted in the highest motility (33 ? 8 %) and velocity (118 ? 9 ?m/s) of spermatozoa after thawing and diluting in swimming medium. We determined that -90 °C is an optimal temperature at which immersing the samples in LN does not affect sperm motility after thawing. The sperm motility of samples immersed in LN before or immediately after the crystallisation point (-16 °C) was 0 %. Motility of spermatozoa cryopreserved with or without a seeding procedure was not significantly different after thawing. Therefore, we hypothesise that supercooling the sample during the conventional freezing procedure is not the main damaging factor during carp spermatozoa cryopreservation.
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Male Sterlet (Acepenser ruthenus) Fertility under Fish Farming Conditions of the Czech Republic
SCHACHERLOVÁ, Jiřina
Currently, fish fertility studies are mainly focused on female fertility. Studies showing fertility of male fish of any kind are missing to a large extent. The main objective of my thesis was to identify what level of fertility is achieved by male sterlet (Acipenser ruthenus) in the conditions of fish farming in the Czech Republic. Male fish from the Vodňany farm where the raised population comes from Russia was compared to male fish from Pohořelice where the raised population comes from Danube. The total comparison test involved 49 pieces of fish. Following stripping to collect semen from male fish, the concentration of sperm (.109.ml-1) was measured in a 1 ml-1 semen sample. Consequently, a sperm count per 1 kg-1 male fish (.109.ml-1) and a sperm volume per 1 kg-1 male fish (ml-1) and a total male fish sperm count (.109) were measured. The highest values of weight identified in male fish was measured in the Pohořelice fish farm. The lowest values of weight was measured in male sterlet of the Vodňany fish farm. The highest as well as lowest volumes of collected semen per 1 kg-1 male fish were identified in Vodňany. However, the average volumes of both cultures were not significantly different. It was concluded by comparison that collected semena volume does not depend on the weight of said fish. Also, the relative fertility was significantly different between male fish of the same population. The highest and lowest sperm count per 1 kg-1 male sterlet were identified in Vodňany. The average minimum and maximum sperm counts per 1 kg-1 male fish were different very much which shows that a greater number of male fish should be used for spawn to ensure sufficient egg fertilization. The average overall fertility values in both cultures are very similar and mainly different in specific specimen of the same culture as well as between those cultures. The results showed that a total sperm count in semen depends on the volume of collected semen. It was further concluded that sterlet fertility does not depend on the size (weight) of fish.
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Cryoconservation of fish sperm in model species - common carp (Cyprinus carpio): the influence of different temperature regimes of cryopreservation on the viability of thawed spermatozoa.
SOCHOROVÁ, Denisa
Influence of temperature and freezing rate on sperm survival after thawing were objective of this study. Motility (percentage of moving sperm), velocity and duration of sperm movement before and after process of freezing were observed in common carp (Cyprinus carpio L.) spermatozoa. Solutions recommended by Kopeika (1986) and Kurokura (1984) were used as a cryoprotective media. Sperm freezing was performed in 0.5 ml straws layed in styrofoam box 3, 6 and 9 cm above the level of liquid nitrogen for 20 min. Temperature changes during process of freezing were recorded inside and outside straws using thermocouple thermometer with miniature probes T type (cuprum ? constantan). At a fi rst level (corresponding to height 3 cm above level of liquid nitrogen) we recorded lowest temperature -170 °C, on a second (6 cm) -110 °C and on a third level (9 cm) -70 °C. Best results of sperm motility after freezing ? thawing were achieved using Kopeika solution and freezing at first level (3 cm above liquid nitrogen) where we reported 27 % of motile spermatozoa and velocity of movement 118 ?m.s-1. Freezing by Kurokura solution resulted in motility 14 % and velocity 76 ?m.s-1 whereas motility of native sperm was 88 % and velocity 136 ?m.s-1.
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