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Analyses of lignans in selected variants of linseed (Linum usitatissimum L.)
DOŠEK, Miroslav
Oilseed flax is one of the traditional agricultural crops. Flax seeds contain, among other beneficial components, lignans, which are polyphenolic substances that provide the plant's defense against biotic and abiotic stress. The main naturally occurring lignan in flax is secoisolariciresinol diglucoside (SDG). Flaxseed represents the richest natural source of lignans, which have been observed to have many positive effects on human health, including antioxidant and other biological activities. One method that can be used to determine SDG is high-performance liquid chromatography with mass spectrometry (HPLC-MS), which was used in this study for the quantitative analysis of SDG. Total polyphenol content (TPC) and antioxidant activity expressed as the ability to quench the DPPH radical were also determined. Correlation analysis was performed to determine the relationships between the studied parameters. Both variety and year had a significant influence on all three studied parameters. The highest average content of polyphenols and SDG was found in the Agram variety, while the highest antioxidant activity was determined in the Floral variety. A significant positive correlation was observed only between the total polyphenol content and the SDG content. The results of this study demonstrate the considerable influence of variety and growing conditions on the content of lignans and the antioxidant potential of flaxseed, which predetermines its health benefits.

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