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Biosyntéza sekundárních metabolitů u Scenedesmus quadricauda v přítomnosti nanočástic
 Kučerová, Dana
The theoretical part of thesis deals with microalgae, primary and secondary metabolism of plants, formation of the reactive oxygen species and origin of oxidative stress. Also it describes secondary metabolites, the characteristic of nanoparticles and impact on microalgae. The practical part deals with the impact of ZnO nanoparticles on secondary metabolism in Scenedesmus quadricauda. At first was observed the impact of nanoparticles on growth of microalgae. Then the content of photosynthetic pigments, total polyphenols, flavonoids and total antioxidant capacity was determined using spectrophotometric method. Also the content of chosen secondary metabolites was detected using HPLC. The growth of microalgae was stimulated by ZnO nanopartlicles during the first 4 days compared to control. Then the inhibition of growth was observed. The content of photosynthetic pigments decreased compared to control in samples with ZnO nanoparticles. Also the change of secondary metabolites, total polyphenols and flavonoids was observed.
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