National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Distribution of S-alleles in Brassica genus
ŽALUDOVÁ, Václava
Self-inkompatibility (SI) is a system how to prevent self-pollinaton in population and enable gene-flow and diversification. SI determines one polymorphic locus S-locus which contains several genes responsible for SI reaction. One of genes lying on the S-locus is the SLG gene and according sequence similarity SLGs are devided into two groups. First group is considered to be domminant and the second recessive. Incompatible lines and five cultivars of Brassica napus, different SLG alleles were cloned and sequenced. Thirty-one sequences of different SLG clones were obtained and phylogenetic tree deduced.Three main groups of SLG sequences are obvious and it seems to have different allelic specificity. Two groups included majority of sequences from SI lines and cultivars suggest two S-alleles very common in Brassica napus. These results were compared with NBCI with different species of Brassica and also with Raphanus sapivus. After cluster analysis assessment was phylogenetic tree created, witch shows a great differences between different SLG classes, but slight differences between Brassica species.
The mechanisms of pollen incompatibility in the Brassicaceae family
Šesták, Petr ; Fíla, Jan (advisor) ; Vosolsobě, Stanislav (referee)
Sporophytic incompatibility (SI) represents one of the systems by which angiosperms prevent pollination by their own pollen or by the pollen from a genetically related plant. It is mostly studied in the Brassicaceae family, mainly due to its agricultural importance. Another reason is that the model plant Arabidopsis thaliana belongs to this family. In the last three decades, advances in molecular biological methods enabled the characterization of a large part of the signalling cascade that leads to the rejection of incompatible pollen. Then, the functions of various cellular components (e.g. cytoskeleton, exocyst or proteasome) involved in the incompatible response to pollination are studied mainly by live cell microscopy. Last but not least, the function of SI under various abiotic stresses was described to reveal their influence on SI mechanisms. The aim of this bachelor thesis is to summarize the new discoveries characterizing the molecular mechanisms of SI in the Brassicaceae family, to describe the processes leading to the germination of compatible pollen grain and to characterize the newly described proteins involved in cellular signalling leading to the rejection of incompatible pollen.
Marker assisted selection in hybrid breeding of oil seed rape
HAVLÍČKOVÁ, Lenka
Marker assisted selection in hybrid breeding of oil seed rape

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