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Role of the 14-3-3 protein in the regulation of G-protein signaling
Řežábková, Lenka ; Obšil, Tomáš (advisor) ; Konvalinka, Jan (referee) ; Bařinka, Cyril (referee)
Univerzita Karlova v Praze Přírodovědecká fakulta Studijní program: Fyzikální chemie Mgr. Lenka Řežábková Studium úlohy proteinů 14-3-3 v regulaci G-proteinové signalizace Role of the 14-3-3 proteins in the regulation of G-protein signaling Disertační práce Školitel: doc. RNDr. Tomáš Obšil, Ph.D. Konzultanti: doc. RNDr. Petr Heřman, CSc. doc. RNDr. Jaroslav Večeř, CSc. Praha, 2012 Abstract The 14-3-3 family of phosphoserine/phosphothreonine-binding proteins dynamically regulates the activity of their binding partners in various signaling pathways that control diverse physiological and pathological processes such as signal transduction, metabolic pathways, cell cycle and apoptosis. More than 300 different cellular proteins from diverse eukaryotic organisms have been described as binding partners for the 14-3-3 proteins. During my Ph.D., I was particularly interested in the role of 14-3-3 proteins in the regulation of G protein signaling pathway. The 14-3-3 proteins affect the G protein signaling via the interaction with negative regulators of G protein cascade - the RGS proteins and phosducin. I employed both biochemical and biophysical approaches to understand how the activity and function of RGS3/14-3-3 and phosducin/14-3-3 complexes are regulated. I solved the low-resolution solution structure of...
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