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Methods of detection of wheat and barley strain of Wheat dwarf virus in vector Psammotettix alienus by PCR-RFLP
Jaňourová, Blanka ; Ripl, Jan ; Kumar, Jiban
Wheat dwarf (causal agent Wheat Dwarf Virus - WDV) is one of the most important cereal diseases in the Czech republic where it can cause huge economic losses in wheat, barley, triticale, rye as well as in oat. Psammotettix alienus is the only known vector of WDV while another way of transmission is unrecognized. This methodology describes a PCR assay for sensitive detection of Wheat Dwarf Virus in Psammotettix alienus, and RFLP assay for barley and wheat strains specific identification.
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