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Expresní profily vybraných genů při vyrůstání kotylárních pupenů hrachu
Psotová, Martina
The diploma thesis was aimed to monitor the expression profiles of selected genes in the growing of cotyledonary buds in pea (Pisum sativum L.). The apical stem was decapitated and 0.5% auxin paste was applied to the stump. From the treated plants cotyledonary buds were collected in intervals 0, 1, 3, 6, 12, 24, 48 and 120 hours. The biological material was analyzed for expression of genes PsDRM1, PsBRC1, PsAUX1, PsPIN1, PsYUC1. A gene expression normalization factor was calculated based on constitutive genes PsEF1-alpha, PsActin and Ps beta-tubulin. Application of auxin to the stem stump prevented the growth of cotyledonary buds. This inhibition of cotyledonary buds was accompanied by increased expression of dormancy markers PsDRM1, PsBRC1 and auxin biosynthesis gene PsYUC1 and decreased expression of the auxin transporter genes PsPIN1 and PsAUX1.
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