National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
Perception of the Cell wall integrity system signals in plant cells
Hercíková, Anna ; Fendrych, Matyáš (advisor) ; Cifrová, Petra (referee)
The cell wall is a key compartment of the plant cell for elementary physiological processes such as cell growth, division, or differentiation, simultaneously protecting the cell from influences of the external environment. The mechanical support of the cell wall maintains the shape of the cell, and at the same time allows it to grow. Thus, the cell wall must not be limiting to cell expansion, but if it becomes too loose, the cell may rupture. Supervised adaptive reorganisation of the cell wall based on external and internal conditions is therefore essential for plant cell, as indicated by the presence of a complex signalling system. The Cell wall integrity (CWI) system represents the set of all mechanisms that together ensure the continuous compactness of the cell wall. My bachelor thesis will discuss the individual components of the CWI system, focusing on the CrRLK1Ls, receptors from the broader Receptor-like kinase (RLK) family.
Preparation of fluorescently labeled substrates and determination of transglycosylation activity in plants
Velínská, Kamila ; Ing.Kristína Kováčová (referee) ; Firáková, Zuzana (advisor)
The bachelor's thesis was focused on preparation of fluorescently labeled oligosaccharides and detection of transglycosylating/heterotransglycosylating activities of enzymes from dicotyledons. As the model organisms nasturtium and parsley were used. The quality and composition of labeled acceptor substrates was monitored by TLC and MALDI-TOF MS. The presence of enzymes in the plant seeds was analysed by SDS electrophoresis and IEF-PAGE. The paper method and HPLC were used for the detection of transglycosylating activities. The obtained results suggest that enzymes catalyzing heterotransglycosylating reactions are in the seeds of studied plants.

Interested in being notified about new results for this query?
Subscribe to the RSS feed.