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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
IEF and HPLC-based methods for efficient bacterial characterization
 Kubesová, Anna ; Šalplachta, Jiří ; Moravcová, Dana ; Horká, Marie
Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease.
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MALDI-TOF MS of Dickeya and Pectobacterium species - Characterization of plant pathogenic bacteria
 Šalplachta, Jiří ; Kubesová, Anna ; Horký, J. ; Horká, Marie
MALDI-TOF MS was applied to reliable characterization of Dickeya and Pectobacterium species in this study. Our results confirm suitability of suggested analytical approach for efficient bacterial differentiation of species based on species specific mass fingerprints. MALDI-TOF MS of intact bacterial cells require minimum sample handling and is less time-consuming compared to conventional methods.
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