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Tolerance Pavlownia tomentosa in vitro k suchu a osmotické zátěži
Zapalač, Radek
This diploma thesis deals with the influence of abiotic stressors on plant growth and their acclimation responses. An in vitro culture experiment was conducted with Paulownia tomentosa plants for 35 days, in which growth parameters (reduction of leaf turgescence, leaf damage, plant height, number of nodes per stem, number of leaves and number of roots) and plant survival, under the treatment of three different osmotics at different concentrations in the medium (20 g/l sucrose – control, 80 g/l sucrose, 50 mM NaCl, 100 mM NaCl, 1.5 % PEG6000, 3 % PEG6000). On days 10 and 30 of the experiment, plant samples were collected for determination of ABA and proline content in leaves. The radioimmunoassay method was used to determine ABA content, while proline content was determined by spectrophotometric measurement. During the first ten days of cultivation, gas samples were also taken from the culture vessels to determine ethylene production, which was subsequently carried out by gas chromatography. The results obtained were statistically and graphically processed and supplemented by photodocumentation.
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