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Chalkbrood desease detection in honeybees
PROKOPOVÁ, Tereza
Chalkbrood disease is a disease caused by the fungal pathogen Ascophaera apis, which attacks and kills bee larvae. It´s most common in weak colonies ,that have a poor cleansing soil and don´t properly remove calcareous agents. The ideal solution is to exchange the bee mother, which will increase the cleansing instinct of the bees, which then completely removes the infection. The aim of my bachelor thesis was to master the methodology of the analysis of the occurrence of Ascosphaera apis using the PCR method, to acquire routine procedures used in the laboratory including DNA isolation and to elaborate a professional research on the topic. In the theoretical part of the thesis I describe the fungal disease of chalkbrood and its influence on the infected bee. Further, I describe in detail methods that are used for the detection of diseases such as DNA isolation, PCR and gel electrophoresis. In the practical part, which I did in the laboratories of the Biotechnology Center, which is part of the Department of Genetics and Plant Production at the Faculty of Agriculture of the University of South Bohemia in České Budějovice, I describe in detail the exact methods used in the detection of this disease and the evaluation of my own results.
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