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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Opportunistic parasites in patients of geriatric facilities
 ČERNÁ, Lenka
The main aim of this work has been the screening of opportunistic intestinal parasites in old people. The older persons may be vulnerable by opportunistic parasites due to the aging of the immune system. Stool samples were collected from patients of different age and sex in a geriatric facility and from the elderly who live in their homes. Light and fluorescent microscopy and molecular diagnostics (PCR) were used as the diagnostic methods. A sedimentation concentration method (M.I.F.C.) was used for the detection of protists, cysts and helmint eggs or larvae and the staining method Miláček et. Vítovec for the detection of cryptosporidia. Staining with calcofuor served for the detection of microsporidial spores. PCR was used for identification of microsporidia species in the stool samples. Only mammalian microsporidia Encephalitozoon cuniculi and Enterocytozoon bienusi were detected in the stool samples. Prevalence of microsporidia in patients of a geriatric facility was 8,3 % and in the second group 18 %. Most positive samples (33%) were in the age category 47 {--} 60 years.
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