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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Molecular detection of Mycoplasma gallisepticum based on whole genome data
 KURÁŽ, Jakub
This thesis reviews the basic knowlegde of a bacteria Mycoplasma gallisepticum and attempts to propose a functional detection protocol to distinguish M. gallisepticum from related species of the genus Mycoplasma. Real time PCR optimalization has refined the specificity and increased the efficiency of detection of selected genomic loci for M. gallisepticum. The functionality and accuracy of the method was verified in laboratory experiments by detecting the occurrence of M. gallisepticum in mixed Mycoplasma samples.
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Evolution of immune response driven by increasing pathogen virulence
 Hančová, Klára ; Vinkler, Michal (advisor) ; Macháček, Tomáš (referee)
Coevolution is relationship between two or more species reciprocally affect each other's evolution. Coevolution is also known as host-parasite interaction where parasite becomes the driving force of host evolution. In an effort to escape the parasite, hosts create different mechanisms that protect themself from infection or minimize its impact. This text is written to acquaint the reader with the host-parasite interaction in response to improving parasite. In systems with myxoma virus and Mycoplasma gallisepticum are described changes of virulence and manipulation with host immune response. Myxomatosis should be known for its increasing virulence because of horizontal transmission. But after many years coevolution between European rabbit (Oryctolagus cuniculus) settled on low value in three different places. Second system represents young evolution between mycoplasma and house finch (Haemorhous mexicanus) which offer opportunity to watch new beggining of defense mechanism against parasite.
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