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National Repository of Grey Literature

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Molecular detection of Mycoplasma gallisepticum based on whole genome data KURÁŽ, Jakub This thesis reviews the basic knowlegde of a bacteria Mycoplasma gallisepticum and attempts to propose a functional detection protocol to distinguish M. gallisepticum from related species of the genus Mycoplasma. Real time PCR optimalization has refined the specificity and increased the efficiency of detection of selected genomic loci for M. gallisepticum. The functionality and accuracy of the method was verified in laboratory experiments by detecting the occurrence of M. gallisepticum in mixed Mycoplasma samples. Detailed record

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