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Design of manipulator of the sample for CCHM microscope
Špaček, Matěj ; Brada, Michal (referee) ; Dostál, Zbyněk (advisor)
In the Laboratory of the Experimental Biophotonics at Brno University of Technology, a new generation of the Coherence-Controlled Holographic Microscope (CCHM) is being developed. Because of the automation of certain microscope procedures, a fully motorized three axis computer controlled manipulator of the sample is to be employed. A fast and accurate lateral positioning in a range of several centimeters is required. For the focusing capability, very precise positioning in the optical axes is required as well. Initially, the basics of microscope manipulator design are presented. Then, commercially available solutions are introduced and compared with special focus on its possible implementation in the new generation of CCHM. However, due to the specific design of the new CCHM, available manipulators are not suitable. Therefore, a new optimized manipulator design is introduced in the second part of the thesis. A selection of its appropriate elements, especially actuators, is based on a review. For the new manipulator design, a technical documentation was made and a testing software was developed. A crucial part of the manipulator was assembled and tested.
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Design of manipulator of the sample for CCHM microscope
Špaček, Matěj ; Brada, Michal (referee) ; Dostál, Zbyněk (advisor)
In the Laboratory of the Experimental Biophotonics at Brno University of Technology, a new generation of the Coherence-Controlled Holographic Microscope (CCHM) is being developed. Because of the automation of certain microscope procedures, a fully motorized three axis computer controlled manipulator of the sample is to be employed. A fast and accurate lateral positioning in a range of several centimeters is required. For the focusing capability, very precise positioning in the optical axes is required as well. Initially, the basics of microscope manipulator design are presented. Then, commercially available solutions are introduced and compared with special focus on its possible implementation in the new generation of CCHM. However, due to the specific design of the new CCHM, available manipulators are not suitable. Therefore, a new optimized manipulator design is introduced in the second part of the thesis. A selection of its appropriate elements, especially actuators, is based on a review. For the new manipulator design, a technical documentation was made and a testing software was developed. A crucial part of the manipulator was assembled and tested.
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