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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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Microscope Image Analysis in LabVIEW
Kotlánová, Markéta ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
Bachelor’s thesis deals with the microscope image analysis and microscope image processing in LabVIEW. The thesis describes the theory of image analysis and image processing, the implementation of the measuring devices and the image acquisition using a camera. In an environment LabVIEW program was created image analysis software. The software is divided into image preprocessing, image processing, image analysis and color image processing. The software allows changing the parameters in used functions.
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Microscope Image Analysis in LabVIEW
Kotlánová, Markéta ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
Bachelor’s thesis deals with the microscope image analysis and microscope image processing in LabVIEW. The thesis describes the theory of image analysis and image processing, the implementation of the measuring devices and the image acquisition using a camera. In an environment LabVIEW program was created image analysis software. The software is divided into image preprocessing, image processing, image analysis and color image processing. The software allows changing the parameters in used functions.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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