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Examination of microdeletion of chromosome Y in male infertile couples
VELÍŠKOVÁ, Taťána
In the last time the most popular topic has becouse infertility. The fertility of men is reduced every year. The cause may be wrong way of living, smoking, use of alcohol and drugs. In addition to can be chemical substances that enter the food and water. Next group of male infertility factors are the effects that lead to warning up the testicles. Optimal temperature is about 32 st. Celsius. People who are wearing tight clothes and have sitting job are proven to be fruitful. In the microdeletion chromosome Y, a long Y chromosome arm is examined on the AZF locus where the AZF, AZFb, AZFc regions are examined. Deletions in these positions are responsible for the failure of sperm formation and maturation and are portable to male offspring. Oligospermia or azoospermia may occur due to the absence of any of the AZFs. The aim of this bachelor thesis was to optimize the multiplex PCR method for the microdeletions in the azoospermic region of the chromosome Y (AZFa, AZFb, AZFc) and to evaluate the acquired data. In theoretical part are characterized diseases relacted to infertility man, for example azoospermic factor, Klinefelter's or Kallman syndrome. In practical part was examined from the scalp of the buccal mucosa 14 sample obtained from the man. All samples was negative, so every men were fruitful with suspicion of one infertile men.
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