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Vliv teploty na vývoj a virulenci entomopatogenní houby Metarhizium anisopliae
 SANDALA, DAVID
Abstract Entomopathogenic fungus M. brunneum has a high potential in integrated pest management against targeted pests. The commercial bio-product Met52 is based on the strain F52 of entomopathogenic fungus M. brunneum. Temperature is the key factor in the inoculums production of strain F52. The optimal temperature for the cultivation of M. brunneum strain F52 is 25 °C. However, the influence of the temperature of inoculum production plays an important role on the growth and spore production during the subsequent cultivation in different temperature conditions. The optimal temperature for radial grows of strain F52 of M. brunneum from different inoculums is 25 °C and subsequently 30 °C. The cultivation of inoculums at 10 °C appears to be negative because at this temperature the strain grows very slow, and spore production is minimal. The morphology phenotype change of inoculums is influenced by 30 °C of cultivation and also this temperature affects the appearance of inoculum central colonies. The highest production of spores was determined from the colonies established from all inoculums cultivated at 25 °C. The highest mortality and the greatest fungus development was recorded at 25 °C and the most virulent to larvae T. molitor were inoculums produced at 15 °C and 20 °C.
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