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Development of a molecular methodology for the identification of insects used for human consumption
MÜLLEROVÁ, Veronika
Insect is often recognized as foodstuff of the future. An important requirement for insect use as a foodstuff is the development of an accurate and reliable method of insect detection. The goal of the bachelor thesis was to develop a molecular method that could be used for detecting the mealworm (Tenebrio molitor), an EU-approved species, in foodstuffs. The experimental part of the thesis included optimalization of mealworm DNA extraction. Specificity test of the method was performer on the superworm (Zophobas morio), which is not approved for consumption in the EU. DNA was extracted using 3 different methods. The optimal method achieving highest DNA yield and purity was DNA extraction with CTAB-PVP. Following DNA extraction, optimalization for PCR mix and cycle was peformed, which included optimalization of primer annealing. Results were visualised by gel electrophoresis. The optimized method is accurate, specific and appropriate for routine detection of the mealworm in foodstuffs.
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Device for measurement and regulation of edible insect breeding conditions
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
This work focuses on the design and realization of a measuring and regulatory system for successful breeding of edible insects with a focus on the mealworm (Tenebrio molitor). For the correct setting of the system, the first part of the work is detected and described the general conditions of insect breeding. The description is mainly focused on the mealworm, its development and basic breeding conditions (temperature, humidity, etc.). This information is further used for the development of breeding equipment, the basic concept of which is further established at work. The device is controlled by espressif's ESP32 microcontroller. The work acquaints with its programming and the connection and programming of basic components for the proposed system. Experimental measurements verify the functionality of measurements and regulation in the breeding box. The regulation is accurate to tenths to hundredths of ° C.Last but not least, the design is compared with existing devices.
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Vliv krmiv na výnos jatečního materiálu hmyzu (Tenebrio molitor)
MUCHKA, Lukáš
Bachelor thesis consists of theoretical and practical part. The theoretical part of this work deals with the consumption of edible insects both in the world and in the Czech Republic. Furthermore, the advantages and disadvantages of the consumption of edible insects and their nutritional value are described. It also includes characteristics of the mealworm (Tenebrio molitor). An important part of the thesis is the practical part, whose aim was to determine the effect of different types of protein feed in the breeding of mealworms. Here their effects on the weight yield of insect slaughter material were compared. The experiment was carried out using four types of protein feed: plant protein (soy), animal protein (dehydrated eggs), complex feed (feed for aquarium fish) and mix of the above feed. Breeding took place in the months of October - December 2019. Two experimental generations of adult insects were bred for egg production. The subsequent egg separation produced separate generations of insects which were fed for fifty-two days. The experiment found that the best results in the breeding of mealworms are achieved by insects, which are fed with a mix of feed in which the largest amount was harvested. Successful breeding of edible insects, however, requires the interplay of several conditions. The choice of feed is one of the important criteria to be addressed. This work is a basic study which can be followed by more detailed experiments.
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Device for measurement and regulation of edible insect breeding conditions
Dvorský, Adam ; Búran, Martin (referee) ; Adámek, Martin (advisor)
This work focuses on the design and realization of a measuring and regulatory system for successful breeding of edible insects with a focus on the mealworm (Tenebrio molitor). For the correct setting of the system, the first part of the work is detected and described the general conditions of insect breeding. The description is mainly focused on the mealworm, its development and basic breeding conditions (temperature, humidity, etc.). This information is further used for the development of breeding equipment, the basic concept of which is further established at work. The device is controlled by espressif's ESP32 microcontroller. The work acquaints with its programming and the connection and programming of basic components for the proposed system. Experimental measurements verify the functionality of measurements and regulation in the breeding box. The regulation is accurate to tenths to hundredths of ° C.Last but not least, the design is compared with existing devices.
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