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Isolation of active substances with antioxidative properties from lignocellulosic waste
 Jurová, Karolína ; Vítová, Eva (referee) ; Kovalčík, Adriána (advisor)
This thesis focuses on isolating active substances with antioxidant properties from lignocellulosic wastes. This work aimed to modify bacterial cellulose (BC) with extracts from grape pomace, which show high antioxidant activity. Grape pomace belongs to the group of lignocellulosic wastes with high content of bioactive compounds such as polyphenols, which contribute significantly to antioxidant efficacy. This work tested the hypothesis that grape extracts can provide antioxidative efficiency to BC. Grape pomace extracts were prepared from grape pomace frozen and lyophilized using a 70% acetone, a 70% ethanol or a 50% ethanol solvent. The highest values of antioxidant activity were determined for the lyophilized extract prepared by extracting grape pomace with 70% acetone. The extracts were further used to modify bacterial cellulose in the form of films (dry BC) and hydrogels (wet BC). It was shown that both forms of BC could be effectively modified using grape pomace extracts, but BC hydrogel achieved higher antioxidant activity compared to BC film. Furthermore, BC with antioxidant activity can be used in various food, cosmetics, and medicine applications.
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