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Studium polymorfizmu DNA u tritikale
Svojsíková, Nikola
Recently known varieties of triticale do not meet milling and baking requirements. The reason is a presence of chromosome R in triticale, which decreases viscoelastic qualities of dough, and a presence of secalins, which decreases technological qualities of grain. Bachelor thesis devotes to detection of high-molecular-weight glutenin subunits (HMW-GS) and low-molecular-weight glutenin subunits (LMW-GS). In total there were 14 genotypes of triticale analyzed in the thesis. DNA markers based on polymerase chain reaction (PCR) were used to identify allelic combinations in loci Glu-A1, Glu-B1 and Glu-D1, which encode high-molecular-weight glutenin subunits, and secalin locus. Alleles Glu-A1a, Glu-A1b, Glu-A1c were detected in locus Glu-A1. Four allelic combinations of glutenin subunits Bx7+By18*, Bx7*+By8, Bx7+By8*, Bx7+By8 were detected in locus Glu-B1. Allele Glu-D1d was detected at two breeding lines. Sec-1 was detected at 12 breeding lines. DNA markers were used to discover allelic composition of locus Glu-A3, which encodes low-molecular-weight glutenin subunits. Alleles Glu-A3a, Glu-A3d and Glu-A3f were not detected at analyzed breeding lines at all.
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