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Využití kultivačních médií pro množení banánovníku metodou in vitro
Kadlecová, Eliška
This work deals with characterization of Musa genus, in vitro cultivation of banana plants and micropropagation of three chosen Kluai Namwa cultivars. Theoretical part comprises of genus Musa taxonomy and particular groups in this genus, then description of all the parts of banana plant, origin of banana growing and cultivars. The world most important cultivars were chosen and described together with their occurence. This chapter includes description of importance and different usage of bananas and world banana trade, too. There can be found overview of the most important pests and diseases of banana plants, ways of multiplication and advantages and difficulties of breeding. The part named Banana plant in vitro deals with specific uses of different aseptic cultures of bananas, it describes some of used practises and their risks. This chapter focuses mostly on micropropagation of banana plants, from establishing the culture to weaning and planting to the field. Third and last part describes the experiment with multiplication of three banana cultivars from Kluai Namwa group (ABB). This experiment took place at Thai university in the year 2015. The goal was to evaluate the suitability of four different cultivation media for individual cultivars and to suggest the best medium for multiplication. Two solid MS media were tested (one with the BA concentration 3 mg.l -1 , the second with 5 mg.l -1 BA) and two liquid MS media with the same concentrations of BA. As the best medium for all three cultivars it turned out to be the liquid one with 5 mg/l BA. But even when this media was used the proliferation of new shoots was low. As the solution the use of fructose as source of energy and multiplication in TIS were sugested.
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