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Experimental Infection of Mice and Ticks with the Human Isolate of Anaplasma phagocytophilum NY-18
KALINOVÁ, Eliška
Anaplasma phagocytophilum is a significant tick-borne pathogen that colonizes granulocytic cells in vertebrate hosts. It is found in North America and in several European countries. It causes severe diseases in domestic animals and potentially fatal disease in humans called human granulocytic anaplasmosis (HGA). The implementation of animal models is necessary due to the increasing prevalence of A. phagocytophilum. This study addresses the transmission of the human isolate of the bacterium A. phagocytophilum NY-18 by two tick species (Ixodes ricinus and Ixodes scapularis) and implements a laboratory model for the transmission of A. phagocytophilum by the tick I. ricinus in mice. The thesis also compares both transmissions and infection dynamics.
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Growth kinetics of the Lyme disease spirochetes in vector ticks \kur{Ixodes ricinus} and \kur{Ixodes scapularis}
VELANOVÁ, Hana
Growth kinetics of Borrelia afzelii CB43 in Ixodes ricinus and Ixodes scapularis were obtained. Based on this data, I. scapularis was marked as capable of acquiring B. afzelii infection. Growth kinetics of B. burgdorferi N40 in I. ricinus was obtained as well with the same outcome, I. ricinus was able to acquire B. burgdorferi infection. Following transmission experiments showed that I. ricinus as well as I. scapularis are able to acquire the infection by B. burgdorferi and B. afzelii. Moreover, we proved that both ticks are able to transmit the infection back to na?ve mice.
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Selected proteolytic aspects as targets to combat ticks and tick borne pathogens
HARTMANN, David
Ticks and tick-borne diseases (TBD) represent a growing global burden for both human and animal health. Tick-host-pathogen interactions have evolved through dynamic processes that accommodated the genetic traits of the hosts, pathogens transmitted, and the vector tick species that mediate their development and survival. As in other parasites, proteases and proteolysis have been found as one of the key factors in this interaction triangle. This thesis is focused on selected proteolytic aspects of tick and tick-borne diseases: (i) processing of host blood as a source of nutrients and energy (hematophagy) as a continuum of the long-term goal of the Laboratory of Vector Immunology, that established the currently accepted model of multienzyme degradation of host blood proteins by ticks (ii) proteases in innate immunity (iii) validation of Babesia proteasome as a potential therapeutic target against the tick transmitted apicomplexan parasites.
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