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Study on interactions of protonated aminoacids with low-molecular weight hyaluronan
Chlumská, Jana ; Kalina, Michal (referee) ; Chytil, Martin (advisor)
This bachelor thesis deals with interactions between hyaluronic acid (HA) of low molecular weight (110-130 kDa) and protonated aminoacids lysine and 6-aminocaproic acid. For investigation of these interactions, methods such as pH-metry, conductance measurement, viscometry and dynamic light scattering were used. The electrostacic interactions between carboxylic group of HA and the aminogroup of the aminoacids are presumed. Proving these interactions would allow us to physically modify HA and further more, using such a system as a carrier of pharmaceuticals. The interactions of protonated lysine with HA were studied in pure water or in the solution of NaCl (c = 15 mmol•dm-3). Interactions of 6-aminocaproic acid were studied only in pure water. Both aminoacids were in the concentration range 0.9-20 mmol•dm-3. The decrease in the viscosity of the solutions indicates the interactions for lysine; for 6-aminocaproic acid the interactions also occured, but not as signifciantly as for the system containing lysine.
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Viscosimetry of hyaluronan-amphiphile systems
Trojan, Martin ; Běťák, Jiří (referee) ; Chytil, Martin (advisor)
This bachelor thesis investigates the interaction between the polysaccharide sodium hyaluronate (HA) and some amphiphilic molecules. As amphiphilic molecules the aminoacids lysine and 6 – aminocaproic acids were chosen. The presence of the carboxylic group on HA and the aminogroup on the aminoacids suggests electrostatic interaction between these two compounds. This supposal offers the possibility to modificate HA and uses it as a new type of a carrier of bioactive compounds, for example medicals. The HA of two molecular weights (1.75MDa and 70kDa) were chosen for the study of the interactions with the aminoacids. Solutions of HA and stock solutions of the aminoacids were prepared for their mutual mixing in the various volume ratios. The solutions and the mixtures were measured in three types of environment: pure water, 0.15M solution of NaCl and in phosphate buffer (PBS) of pH 6. The interactions were investigated by means of reometry. Our results show, the presence of interactions between HA and the aminoacids mainly in the system consisting the 1.75 MDa HA and lysine in water. As a result of the interactions, a decrease in the viscosity of the mixtures comparing to the HA solutions was observed.
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DYMAMESI 2016 - proceedings
Zolotarev, Igor ; Pešek, Luděk
Proceedings of the Colloquium DYNAMICS OF MACHINES AND MECHANICAL SYSTEMS WITH INTERACTIONS (DYMAMESI), that was held in the Institute of Thermomechanics in Prague, March 1 - 2, 2016 deal with problems structural and multibody dynamics, in coupled interacting systems as aero-elasticity, hydro-elasticity, biomechanics, systems with feedbacks and mechatronics. The published papers was divided into the two parts: Interactions - vibration of dynamic systems coupled with surroundings, mechanical systems with feedbacks, mechatronic active elements, measurement methods and identification in interaction problems, interactions in biomechanics, aero- and hydro-elasticity in rotational engines (bearings, blades, sealings, spaces), analytical and numerical methods in solutions of interactions and Dynamics of Machines - dynamics and vibrations of mechanical systems and solids, non-linear systems - stability, bifurcations, chaos, damping technologies - materials, active elements, methods, identification, vibrodiagnostics, fatigue, computational mechanics and experiments.
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Community of phytophilous benthos in fishponds
ZIKMUND, Hynek
Community of phytophilous macrozoobenthos (PM) represents an important component of fishponds ecosystems concerning biomass as well as essential part of food webs. There are evident linkages between PM and water macrophytes. Their influence on PM is the main topic of many scientific papers which state three main water macrophyte characters with the strongest impact on PM - coverage, biomass and morphological complexity. The main aim of this diploma theses was to analyse linkages between four different water macrophyte species and PM in eight different fishponds devided into two groups according their localisation- třeboňské a rakouské. Water macrophyte species have any influence on PM abundance in both fishpond groups. Water macrophyte species have significant influence on biomass of PM in třeboňské and rakouské fishponds analysed together and also in both fishpond groups analysed separately. PM biomass was significantly higher in Glyceria maxima during first sampling period in both fishpond groups. Water macrophyte species have significant influence on PM diversity in třeboňské and rakouské fishponds analysed together and in rakouské fishponds group analysed separately. Just one variable, water macrophyte species, was analysed in my diploma thesis, but some others abiotic and biotic variables influence phytophilous macrozoobenthos community in fishponds littoral zones as well. That´s why conclusions of this thesis should be interpreted circumspectly.
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DYMAMESI 2014 - proceedings
Zolotarev, Igor ; Pešek, Luděk
Proceedings of the Colloquium DYNAMICS OF MACHINES AND MECHANICAL SYSTEMS WITH INTERACTIONS (DYMAMESI), that was held in the Institute of Thermomechanics in Prague, November 25 - 26, 2014 deal with problems structural and multibody dynamics, in coupled interacting systems as aero-elasticity, hydro-elasticity, biomechanics, systems with feedbacks and mechatronics. The published papers was divided into the two parts: Interactions - vibration of dynamic systems coupled with surroundings, mechanical systems with feedbacks, mechatronic active elements, measurement methods and identification in interaction problems, interactions in biomechanics, aero- and hydro-elasticity in rotational engines (bearings, blades, sealings, spaces), analytical and numerical methods in solutions of interactions and Dynamics of Machines - dynamics and vibrations of mechanical systems and solids, non-linear systems - stability, bifurcations, chaos, damping technologies - materials, active elements, methods, identification, vibrodiagnostics, fatigue, computational mechanics and experiments.
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Experiential learning and interactive training projects in impact on pupils in younger school age.
KALMUSOVÁ, Ivona
The work addresses the importance of learning by experience for the development of education in elementary school pupils. The theoretical part of the work analyzes the current situation in the Czech education system, the directions of innovation efforts, and focuses on other opportunities and challenges that come together with modernization of the Czech education. The research primarily pursues an analysis of teachers' view to benefits of interactive educational projects - i.e. their objectives, focus, content and concept of events - in this particular case, interactive exhibits which complement the current system of education. It simultaneously monitors the activity and awareness of questioned teachers in the area of non-traditional methods of education.
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Gravity models and their use in the geographic research on the example of the settlement system of the Vysočina county
BLAŽEK, Jan
This thesis deals with gravitational models which are concerned with interactions between different objects. Thesis applies to these models to the settlement system of Vysočina county. This research discovers the positives and negatives of the models in terms of their application on the particular settlement system. The centres ORP of the Vysočina county are profited for this research. The first part of this thesis deals with interactions between these centres ORP, in light of the theoretical spatial relationships, the real direct bus connections and real commuting to work. In the second part the thesis focuses on the regionalization of the Vysočina county. The regionalization is defined on the theoretical calculations of the Reilly model, as a real gravitational model, a real commuting to work. The requisite data for the analyse were calculated on the basis of the formulas or were taken from the database of the Czech Statistical Office. The maps, which represent the analyzed data, make also part of this thesis.
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The class climate of the first-year of secondary schools depending on the adaptation course
PYTLÍKOVÁ, Anna
The work deals with the comparison of classes that have taken part in an adaptation course to those that have not. The theoretical part describes a class as a small social group and refers to its structure. It also deals with group dynamics and explains developmental stages of a small social group. Further, the theoretical part of the thesis focuses on class climate, environment and atmosphere. It describes the adaptation course and defines its aims as well. The practical part of the thesis contains a description and an analysis of questionnaires that were assigned to the first year classes of the four-year grammar schools in the early days of the school year 2011/2012 and in the beginning of November the same year. Based on the analysis of questionnaires, this part also deals with identifying differences among these classes. It points out that the differences among these classes are small but, nevertheless, the adaptation courses are of benefit to the whole class.
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INTER-ACTION
KRAUSOVÁ, Kateřina
The Thesis deals with interactions of the trachet and artist. The theoretical section contains selected educational-psychological perspective oninteraction, complemented by a sociological perspective in the kontext of the changing times of the communication world by John Thompson. The practical part narrows to specifically interact using touch, and tactile perception of visual artifacts. The real impulse comes from the work of Jan Svankmajer and interactive exhibitions of Peter Nikl. The result of this work is a set of interactive, visual haptic objects for presentation in the form of a separate exhibition.
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